Study on the inheritance of very dwarfness in Citrus and Poncirus and its use for breeding

柑橘、枳属极矮化遗传及其育种研究

基本信息

批准号：
02660036
负责人：
WAKANA Akira
金额：
$ 1.41万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660036/
关键词：
Poncirus Cirus Very dwarfness Gibberellin single reseccive dwarf gene Rootstock Precocious flowering Modified gene 矮性単劣性遺伝子細胞質遺伝不完全優性

项目摘要

Genetical, morphological and histological studies were carried out to demonstrate the characteristics of various types of dwarf seedlings occurred in Citrus and Poncirus. When the polyembryonic cultivars were used as a pistilate parent, zygotic seedlings were determined by isozyme analysis. In the seedlings from self-pollination of 'Flying Dragon', normal and Flying Dragon-shaped seedlings and very dwarf seedlings segregated at the ratio 3:1, indicationg that 'Flying Dragon' is heterozygous for single recessive dwarf gene and that very dwarf seedlings are homozygous for it. This assumption were supported by the evidence that in crosses between citrus cultivars and 'Flying Dragon', normal seedlings were produced.Since the growth of the very dwarf seedlings was promoted by 5-25ppm gibberellin treatment and grafting on normal trifoliate orange, this reseccive dwarf gene is considered to inhibit gibberellin synthesis. The morphological and histological charactors of very dwarf seedlings we … More re very different from those of normal and Flying- Dragon-shaped seedlings.The elongated dwarf seedlings on normal trifoliate orange could be divided into normal and Flying Dragon-shaped types, indicating that Flying Dragon dwarfness was controlled by Flying Dragon dwarf gene. However, we could not clearly resolved the mechanisum of the ingeitance.Since selfed 'Chimotto' produced normal and dwarf seedlings at the ratio 1:3 and since citrus and Poncirus cultivars pollinated with 'Chimotto' produced them at the ratio 1:1 and 15:1, 'Chimotto' type dwarfness is considered to be controlled by dominant major gene which interacts with modified genes. 'Bouquet' type dwarfness is thought to be controlled by single dominant gene which interacts with lethal gene, since seedlings obtained from citrus cultivars pollinated with 'Bouquet' could be divided into dwarf and normal types at the ratio 2:1. Further study for these hypothesis is now in progress.Lastly, the present results suggest that use of 'Flying Dragon' as a pistilate parent is sutable for breeding dwarf rootstocks for citrus, since 10% of zygotic seedlings from self-pollination of 'Flying Dragon' showed precocious flowering. Less

进行了遗传，形态学和组织学研究，以证明在柑橘和多布尔斯中发生了各种类型的矮幼苗的特征。当将多胚品品种用作雌二二酸幼体时，通过同工酶分析确定了二聚幼苗。在“飞龙”的自花授粉中的幼苗中，以3：1的比例隔离幼苗，非常矮的幼苗，表明“飞龙”是单一隐性矮矮人的杂合子，对于单个隐性矮矮人的基因而言，这是非常矮的幼苗对此的纯种幼苗。这一假设得到了以下证据：在柑橘品种和“飞龙”之间的十字架中，产生了正常的幼苗。由于非常矮幼苗的生长是通过5-25ppm gibberellin治疗促进的，并在正常的三叶橙橙色上嫁接了，这种重新检测的Dwarf Gene被认为可以抑制Gibberillellellins onseess anttheSiss。非常矮幼苗的形态学和组织学特征与正常和飞龙形幼苗的恢复更为不同。普通三叶橙上的细长矮幼苗可以分为正常和飞行的龙形类型，表明飞龙矮人是由飞行的龙矮人基因控制的。然而，我们无法清楚地解决食材的机械分歧。从自我的“ Chimotto”中产生了正常的和矮幼苗，并且由于柑橘和Poncirus品种用“ Chimotto”授粉的“ Chimotto”授粉了“ Chimotto”以1：1和15：1的比率产生了它们，因此由“ Chimotto'类型的dwarfness相互作用。 “花束”类型矮人被认为是由与致命基因相互作用的单个显性基因控制的，因为从柑橘品种获得的幼苗可以将“花束”授粉的幼苗分为矮人和以比例为2：1的正常类型。目前正在进行这些假设的进一步研究。目前的结果表明，使用“飞龙”作为pistilate父母是可持续的，可为柑橘繁殖矮dwarf dootstocks，因为从“飞龙”的自我剥削中，有10％的zygotic幼苗表现出早期的开花。较少的