IDENTIFICATION OF LINKAGE GROUPS USING TORISOMIC SERIES IN CITRUS

使用柑橘中的异构序列鉴定连锁群

基本信息

批准号：
05660035
负责人：
WAKANA Akira
金额：
$ 1.41万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Because of the presence of the problems of long juvenility, nucellar embryogenesis and so on, genetic analysis in Citrus was difficult. Recently, however, linkage relationship of molecular genes has been reported by several workers, as the methods for molecular genetics develops. To use these genes as genetic markers and to develop the chromosome map for Citrus, it is essential to show which chromosome has them. The purpose of the present studies is to produce trisomic series using triploid citrus, and to identify the chromosome containing isozyme genes using trisomic series.Both cross combinations 2X*3X and 3X*2X produced trisomic seedlings with chromosome number of 2n=19. But the frequency of trisomic seedlings obtained was very low. In the case of 2X*3X almost all seedlings obtained were dipliod with 18 chromosomes, and a few aneuploid seedlings were obtained. In the case of 3X*2X relatively a large number of aneuploid seedlings were obtained, but number of trisomic seedlings obtained was small. The undeveloped embryos within the imperfect seeds obtained from both crosses were cultured in vitro, but we failed to produce a alrge number of seedlings from these embryos. Only 10 seedlings obtained through the embryo culture were diploid. We improve the culture now.In the present study, we obtained 18 trisomic seedlings. However, growth rate pf these seedlings were very low and some of them were dead Hence, we could not performed isozyme analysis using these seedlings. The morphology of chromosomes of trisomic seedlings was observed by several methods, but we could not identified the trisomic chromosome. Based on the present study, further research will be cont inued.

由于存在长幼年，核胚发生等问题，因此柑橘的遗传分析很困难。然而，最近，随着分子遗传学方法的发展，几位工人已经报道了分子基因的连锁关系。要将这些基因用作遗传标记并开发柑橘的染色体图，必须证明哪种染色体具有它们。本研究的目的是使用三倍柑橘类产生三化序列，并使用三叶综合级数鉴定含同工酶基因的染色体。跨组合2x*3x*3x*2x*2x产生的三叶念珠幼苗，染色体数量为2n = 19。但是，获得的三体幼苗的频率非常低。在2x*3倍的情况下，几乎所有获得的幼苗都是具有18种染色体的齿轮，并且获得了一些非整倍幼苗。在3x*2x的情况下，获得了大量的非整倍幼苗，但获得的三晶幼苗数量很少。在体外培养了从两个十字中获得的不发育种子中未开发的胚胎，但我们未能从这些胚胎中产生数量的幼苗数量。仅通过胚胎培养获得的10个幼苗是二倍体。我们现在改善了培养物。在本研究中，我们获得了18种三叶幼苗。但是，生长速率PF这些幼苗非常低，其中一些幼苗是死亡的，因此我们无法使用这些幼苗进行同工酶分析。通过几种方法观察到了三化幼苗的染色体的形态，但我们无法鉴定三异构体染色体。基于本研究，将进行进一步的研究。