Fundamental Research on Laser Irradiation Effect on Cell Biology

激光照射对细胞生物学影响的基础研究

基本信息

批准号：
02045006
负责人：
FUJII Kan-ichi
金额：
$ 3.97万
依托单位：
Faculty OF ENGINEERING, IBARAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

The research results obtained by this International joint project are summarized in three items as follows.(1) Success of Visualized quantitative measurements of antibody and its application to decision of production rate of protein.(2) Success of preliminary qualitative measurements of small particles in a solvent by Ramman scattering method with an intent of sorting cancer cells.(3) A model of revival of cancer cell by laser irradiation was proposed and its experimental verification based on comparison of Ramman scat tering with optogalvanic laser spectroscopy is in progressing.The result (1) is based on the ILISPOT (Immunofluorescence Linked Immunospot Assay) method which enable the quantitative measurements of protein. The antibody bound to specific protein is directly combined with a suitable dye.the dye is excited by the light irradiated from the light-source contained in a fluorescence microscope. The fluorescence, emitted from the dyed-antibody id taken into a video camera, bei … More ng measured the protein quantitatively from the light emission intensity and the cross-section of emission spot. This is due to Dr.Amano's original investigation, being it major part of his Ph.D. thesis at The University of Alabama at Birmingham, U.S.A.. In further investigation, we would like to use a new linker-arm containing F as well as He-Cd^+ white-light laser as the light source of the fluorescence microscope. It is expected to improved the s/n ratio by this means.The result (2) is based on Raman scattering laser spectroscopy like in a cell sorter. It can be suggested that an optimal wavelength for exciting individual inner organ and a specific emission spectra from it would exist as the inner organs have different colors. So, using the He-Cd^+ white light laser consisting of three primary colors, it is expected that three kinds of cells can be sorted simultaneously. As a preparation for this aim, Ramman spectra were observed in acetone and nitrobenzine, thereby measuring system was accomplished. Cells would be examined in the near future at next stage.The result (3) is a new subject born on the way to the goal of this project. According to the Generation mechanism of the cancer cell proposed in this project, the energy state of a cancer cell is higher than that in a healthy cell, corresponding to a metastable state in an atom which is forbidden to return to the ground state Via optical transition. If the metastable state can be released via excitation to an upper level by a laser irradiation, then to the ground state via optical transition, the cancer cell can return to a healthy cell. For the verification to this hypothesis, it is necessary to detect the fluorescence emitted by the transition from higher level than metastable state onto the ground state. Less

国际关节获得的研究结果如下三个项目。在进行的程序中，带有光能激光光谱的方法。荧光中包含的光源辐照的光线，染色的抗体ID被带到摄像机中，从光发射强度和发射点的横截面中进行了定量。 Amano的原始调查是他在美国伯明翰的Alabama博士学位的主要部分荧光。由三种主要颜色组成，可以同时对三种细胞进行分类，因为Asaspectra是观测器和硝基苯，从而在下一阶段进行了测量系统。新的主题是这个项目的目标。我可以健康的细胞，以验证这一假设，必须检测到比稳态到基态的更高水平的过渡