Fundamental of optogalvanic laser spectroscopy based on relaxation oscilator detection and its applications.

基于弛豫振荡器检测的光电激光光谱学基础及其应用。

基本信息

批准号：
02452087
负责人：
FUJII Kan-ichi
金额：
$ 4.22万
依托单位：
Ibaraki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

This research subject has been carried out for two years. The investigation contains two aspects ; one of them is further research of optogalvanic spectroscopy itself which was newly developed with an intent of getting higher sensitivity of detecting optogalvanic effect. The other is its application to cell biology.At the part of fundamental investigation, the measurement of optogalvanic signal targeted to the negative glow plasma was expanded to all plasma in a glow discharge like the positive column, Faraday dark space, anode glow, and the cathode dark space. By this means, a high quality measurement of plasma properties became hopeful. During this experiment, unusual optogalvanic signal was detected in the cathode dark space ; i. e., the polarity of the signal was reversed. So, experiments have been still continuing in order to confirm its reproducibility of the new result.At the part of application, the investigation for sorting cancer cell by means of autofluorescence which will be emitted from each cancer cell by the irradiation of laser beam of suitable wavelength was started. This method is the one included in the Raman spectroscopy. The authors started to detect some Stokes line from a mineral such as AgCl particle, and biomaterials like milk, vegetable juice as well. Our final aim is to detect an anti-Stokes line and so, the experiments are being powerfully continued.

该研究主题已经进行了两年。调查包含两个方面。其中之一是对光合光谱法本身的进一步研究，该研究是新开发的，目的是获得更高的敏感性，以检测光能效应。另一个是其在细胞生物学上的应用。在基本研究的一部分中，针对负闪光血浆的optogalvanic信号的测量在闪闪发光的排放中扩展到所有等离子体，例如正柱，法拉第黑暗空间，阳极发光，阳极发光和阴极黑暗空间。通过这种方式，对等离子体特性的高质量测量变得充满希望。在此实验中，在阴极的黑暗空间中检测到异常的光藻信号。我。例如，信号的极性逆转。因此，为了确认其新结果的可重现性，实验仍在继续进行。在应用的一部分中，通过自动荧光对癌细胞进行分类的研究将通过每个癌细胞从每个癌细胞中发出，通过启动合适波长的激光束的照射。该方法是拉曼光谱中包含的方法。作者开始从矿物（例如AGCL颗粒）和牛奶，蔬菜汁等生物材料中检测出一些stokes线。我们的最终目的是检测一条反stokes线，因此，实验正在有力继续。