Fundamental of optogalvanic laser spectroscopy based on relaxation oscilator detection and its applications.

基于弛豫振荡器检测的光电激光光谱学基础及其应用。

基本信息

批准号：
02452087
负责人：
FUJII Kan-ichi
金额：
$ 4.22万
依托单位：
Ibaraki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

This research subject has been carried out for two years. The investigation contains two aspects ; one of them is further research of optogalvanic spectroscopy itself which was newly developed with an intent of getting higher sensitivity of detecting optogalvanic effect. The other is its application to cell biology.At the part of fundamental investigation, the measurement of optogalvanic signal targeted to the negative glow plasma was expanded to all plasma in a glow discharge like the positive column, Faraday dark space, anode glow, and the cathode dark space. By this means, a high quality measurement of plasma properties became hopeful. During this experiment, unusual optogalvanic signal was detected in the cathode dark space ; i. e., the polarity of the signal was reversed. So, experiments have been still continuing in order to confirm its reproducibility of the new result.At the part of application, the investigation for sorting cancer cell by means of autofluorescence which will be emitted from each cancer cell by the irradiation of laser beam of suitable wavelength was started. This method is the one included in the Raman spectroscopy. The authors started to detect some Stokes line from a mineral such as AgCl particle, and biomaterials like milk, vegetable juice as well. Our final aim is to detect an anti-Stokes line and so, the experiments are being powerfully continued.

该研究课题已进行了两年。调查内容包括两个方面；其中之一是对光原光谱本身的进一步研究，这是新开发的，旨在获得更高的检测光原效应的灵敏度。另一个是其在细胞生物学中的应用。在基础研究部分，针对负辉光等离子体的光电流信号的测量扩展到所有辉光放电中的等离子体，如阳极柱、法拉第暗空间、阳极辉光和阳极辉光。阴极暗空间。通过这种方式，等离子体特性的高质量测量变得充满希望。实验过程中，在阴极暗区检测到了异常的光电流信号；我。即，信号的极性颠倒了。因此，实验仍在继续，以确认新结果的可重复性。在应用部分，研究通过自体荧光来分选癌细胞，自体荧光是通过合适的激光束照射从每个癌细胞中发出的。波长开始。该方法是拉曼光谱中包含的方法。作者开始从 AgCl 颗粒等矿物质以及牛奶、蔬菜汁等生物材料中检测到一些斯托克斯线。我们的最终目标是检测反斯托克斯线，因此实验正在有力地继续进行。