Isolation and Identification of Cartilage-derived Factor (CDF) and its Precursor.

软骨衍生因子（CDF）及其前体的分离和鉴定。

• 批准号: 01580163
• 负责人: HIRAKI Yuji
• 金额: $ 1.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01580163/
• 关键词: Cartilage-derived factor; chondrocyte; chondromodulin-I; cell differentiation; cell growth; fibroblast growth factor; extracellular matrix; endochondral bone formation; cDNAクロ-ニング; 精製; アミノ酸配列; プロテオグリカン合成

Besides known hormones and growth factors, some functional matrix components have been suspected to play important roles in the control of chondrogenic expression. Here I report the structure and bioactivity of 25 kDa glycoprotein (chondromodulin-I, ChM-I) as a tissue-specific functional matrix component identified and cloned for the fist time. ChM-I purified from fetal bovine cartilage markedly stimulated DNA synthesis of cultured growth-plate chondrocytes in the presence of basic Fibroblast Growth Factor (FGF). Bovine ChM-I cDNA revealed that mature Ch-M-I consists of 121 amino acids with three possible glycosylation sites and is coded as the C-terminal part of a larger precursor. On northern blot analysis, expression of ChM-I mRNA was observed only in cartilage.

除了已知的激素和生长因子外，还怀疑某些功能性基质成分在控制软骨表达中起重要作用。在这里，我将25 kDa糖蛋白（软骨蛋白-I，CHM-I）的结构和生物活性作为固定时间鉴定并克隆的组织特异性功能基质成分。在存在碱性成纤维细胞生长因子（FGF）的情况下，从胎牛软骨中纯化的CHM-1明显刺激了培养的生长板软骨细胞的DNA合成。牛CHM-1 cDNA表明，成熟的CH-M-I由121个具有三个可能的糖基化位点的氨基酸组成，并被编码为较大前体的C末端部分。在北印迹分析中，仅在软骨中观察到CHM-I mRNA的表达。

项目成果

Yuji Hiraki: "Molecular cloning of a new class of cartilageーspecific matrix,chondromodulinーI, which stimulates growth of cultured chondrocytes." Biochem.Biophys.Res.Commun.(1991)

Yuji Hiraki：“新型软骨特异性基质软骨调节蛋白-I 的分子克隆，可刺激培养的软骨细胞的生长。”(1991)

Hiroyuki Inoue: "Stimulation of cartilageーmatrix proteoglycan synthesis by morphologically transformed chondrocytes grown in the presence of fibroblast growth factor and transforming growth factorーbeta." J.Cell.Physiol.138. 329-337 (1989)

Hiroyuki Inoue：“在成纤维细胞生长因子和转化生长因子-β 存在下生长的形态转化软骨细胞刺激软骨基质蛋白多糖的合成。”J.Cell.Physiol.138（1989）。

H.Inoue,Y.Kato,M.Iwamoto,Y.Hiraki,M.Sakuda,F.Suzuki: "Stimulation of cartilageーmatrix proteglycan synthesis by morphologically transforamed chondrocytes grown in the presence of fibroblast growth factor and transforming growth factorーbeta" J.Cell.Physiol.

H. Inoue、Y. Kato、M. Iwamoto、Y. Hiraki、M. Sakuda、F. Suzuki：“在成纤维细胞生长因子和转化生长因子-β 存在下生长的形态转化软骨细胞刺激软骨基质蛋白聚糖的合成“ J.Cell.Physiol。

Yuji Hiraki: "Molecular cloning of a new class of cartilageーspecific matrix,chondromodulinーI,which stimulates growth of cultured chondrocytes." Biochem.Biophys.Res.Commun.(1991)

Yuji Hiraki：“新型软骨特异性基质软骨调节蛋白-I 的分子克隆，可刺激培养的软骨细胞的生长。”(1991)

Yuji Hiraki: "Differential modulation of growth and phenotypic expression of chondrocytes in sparse and confluent cultures by growth factors in cartilage." J.Bone Min.Res.5. 1077-1085 (1990)

Yuji Hiraki：“软骨中生长因子对稀疏和汇合培养物中软骨细胞生长和表型表达的差异调节。”