A Study of Autoreactive T Cells in Drug-Induced Autoimmune Skin Disease

药物引起的自身免疫性皮肤病中自身反应性 T 细胞的研究

基本信息

批准号：
01570570
负责人：
IKEZAWA Zenro
金额：
$ 1.34万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570570/
关键词：
Sulfhydril drug GVHD-like drug eruption (reaction)IFNgamma TNFalpha Epidermal Langerhans cell HLA-DR of keratinocyte BP antigen of keratinocyte Slot blot hybridization TNFα 角化細胞のBP抗原 SH薬剤皮膚GVHD様変化薬剤特異的TCPR IL-2反応性試験 SH薬剤パルス脾

项目摘要

1) In the drug eruptions, which were classified to the subgroup of acute cutaneous GVHD (graft-verusus-host disease) type from the clinicohistopathological findings, we observed the dominant infiltration of CD8+ T cells in the epidermis, depletion/reduction of CD1+ epidermal Langerhans cells and remarkable expression of HLA-DR/ICAM-1 on the kerationcytes. These findings are the same as acute cutaneous GVHD itself appeared after bone marrow transplantation and blood transfusion, indicating that essentially the same pathomechanism is involved in the both conditions, and also that enhancement of T cells with cytotoxic activity and the inflammatory cytokines such as IFNgamma and TNFalpha may play an important role in development of GVHD itself and GVHD-like reaction by drugs.2) In the footpad reactions by local transfer of sulfhydril drug, tiopronin (TP)-sensitized lymph node cells (LC) and spleen cells (SC), IFNgamma and TNFalpha were also required for the full induction of GVHD-like reactions.3) We have already revealed that IFNgamma, which is released from the activated T cells, enhances the expression of bullous pemphigoid (BP) antigen on the cultured keratinocytes by flow cytometory. In this study we revealed the enhanced expression of BP antigen in the messenger RNA levels by slot blot hybridization. These results reconfirmed the possibility that cytokines such as IFNgamma from the activated T cells might be involved in development of autoimmune bullous pemphigoid.

1）在药物喷发中，该药物被分类为临床组织病理学发现的急性皮肤GVHD（移植物 - 宿主疾病）类型的亚组，我们观察到表皮中CD8+ T细胞的显性浸润，CD1+表皮降低/降低CD1+ Ectermeral的降低/还原Langerhans细胞和HLA-DR/ICAM-1在Kerationcytes上的显着表达。这些发现与骨髓移植和输血后出现了急性皮肤GVHD本身，表明两种情况中基本上涉及相同的病理机制，并且还具有细胞毒性活性和炎性细胞因子（如Ifngamma和ifngamma和ifngamma and ifngamma and ifngamma and ifngamma and ifngamp and and the the complocy tnfalpha可能在GVHD本身的发展和药物的GVHD样反应中发挥重要作用。2）在植入反应中，硫化药物的局部转移TIORPRONIN（TP）敏感性淋巴结细胞（LC）和脾细胞（SC）（SC）（SC）通过局部转移。，还需要为GVHD样反应的全部诱导，还需要Ifngamma和Tnfalpha。流式细胞图。在这项研究中，我们揭示了通过插槽印杂交在使者RNA水平中BP抗原的表达增强。这些结果重新确认了活化T细胞等细胞因子（例如IFNGAMMA）可能参与自身免疫性大肠杆菌的发展。