Serological Diagnosis and Seroepidemiological Study of Q Fever.

Q热的血清学诊断和血清流行病学研究。

基本信息

批准号：
01570240
负责人：
ODA Hiroshi
金额：
$ 0.32万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

1. The growth of Q fever agent (Coxiella burnetii) in vivo and in vitro : Treatment of mice with cyclophosphamide facilitated the growth of Coxiella burnetii so effectively that isolation of the organism became easier. The growth of this rickettsia in cultured cells differed markedly according to the type of cells and the species of animals from which the cells derived.2. Preparation of antigens for serological diagnosis of Q fever : Coxiella burnetii, strain TK-1, in phase I propagated well in yolk sacs of embryonated chicken eggs, and we could obtain enough antigen for serological tests. Phase II antigen of Coxiella burnetii was tried to make by serial passage in yolk sac, and we attained to the 20th passage. On the other hand, trichloroacetic acid-treated phase I antigen reacted strongly with anti-phase II antibody. Therefore this chemically modified antigen must be useful for serological diagnosis.3. Serological diagnosis of Q fever : Immunofluorescence technique was adequate for laboratory examination of Q fever from the viewpoints of rapidity, sensitivity and practicality. Western immunoblotting method was also adequate to check the specificity of the antibody against Coxiella burnetii in reference to 60 kilodalton common antigen.4. Seroepidemiological study of Q fever : Serological examination of bovine and human sera for antibodies against Coxiella burnetii was carried out by the immunofluorescence technique. About 30% of the cows examined were antibody-positive. Sera from two veterinarians also had antibody against C. burnetii. These results suggest that the Q fever agent has spread widely in Japan. Therefore Q fever must be added to the list of differential diagnosis of unknown fever.

1。体内和体外的Q发烧剂（Coxiella burnetii）的生长：用环磷酰胺处理小鼠，促进了Coxiella burnetii的生长，因此有效地隔离了生物体的分离变得更加容易。根据细胞的类型和细胞衍生的动物种类，这种立克人士在培养细胞中的生长明显不同。2。抗原用于Q热的血清学诊断的抗原：在胚胎鸡蛋的蛋黄囊中很好地传播了coxiella burnetii，Tk-1菌株TK-1，我们可以获得足够的抗原进行血清学检查。考克斯·伯内特（Coxiella burnetii）的II期抗原试图通过蛋黄囊中的串行通道制作，我们达到了第20通道。另一方面，三氯乙酸处理的I期抗原与抗相期II抗体反应强烈。因此，这种化学修饰的抗原必须对血清学诊断有用3。 Q热的血清学诊断：免疫荧光技术足以从速度，敏感性和实用性的角度检查Q发烧的实验室检查。西方免疫印迹法也足以检查抗牛coxiella burnetii的抗体特异性，参考60千洛达尔顿公共抗原4。 Q发烧的血清ePIDEMIologicy研究：对牛牛burnetii的牛和人血清的血清学检查是通过免疫荧光技术进行的。检查的奶牛中约有30％是抗体阳性的。来自两名兽医的血清还具有针对C. burnetii的抗体。这些结果表明，Q发烧剂已在日本广泛传播。因此，必须将Q发烧添加到未知发烧的鉴别诊断列表中。