Cellular mechanism of stroke - Characteristics of astroglia from the brain of stroke-prone SHR

中风的细胞机制 - 易发生中风的 SHR 大脑星形胶质细胞的特征

基本信息

批准号：
63570165
负责人：
NARA Yasuo
金额：
$ 1.34万
依托单位：
Shimane Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

Astroglia cells were separated from whole brains of fetal stroke-prone SHR(SHRSP), stroke-resistant SHR(SHRSR) and Wistar Kyoto rats(WKY). Over 95% of the population in all strains was positively stained with antiglial fibrillary acidic protein. There was no morphological change in cultured astroglia cells among three strains. The proliferative activity of astroglia cells was significantly greater in SHRSP and SHRSR than that of WKY. When quiescent astroglia in G_1 phase were exposed to fetal calf serum, a linear increase in [^3H] thymidine was observed after a 9-12h lag period. Although the length of lag period did not seem to be different among three strains, the rate of thymidine incorporation in SHRSP cells was greater than that into WKY cells. SHRSR cells showed the intermediate incorporation between SHRSP and WKY. Similar results were observed in cultured vascular smooth muscle cells from SHR and WKY. Total cholesterol levels in SHRSP and SHRSR were significantly decreased compared to that of WKY. The activities of cholesterol biosynthesis from [^<14>C] acetate or [^<14>C] mevalonate were compared among three strains. The formation of cholesterol from acetate was decreased in SHRSP and SHRSR, however there was no difference in cholesterol biosynthesis from mevalonate among three strains. Astroglia has an alpha receptor and Ca^<++> level in the cell was increased by an alpha-agonist. Cellular Ca^<++> level was dose-dependently increased after treatment of phenylephrine (10^<-5>-10^<-7>M) in three strains and the responsiveness to the stimulation was higher in SHR than that of WKY. These abnormalities, noted also in cultured vascular smooth muscle cells may be of rather general phenotype in SHR.

将星形胶质细胞与胎儿中风的全脑（SHRSP），中风SHR（SHRSR）和Wistar Kyoto大鼠（WKY）分离。超过95％的人群在所有菌株中用抗纤维纤维酸性蛋白呈阳性染色。在三种菌株中，培养的星形胶质细胞没有形态学变化。 SHRSP和SHRSR的星形胶质细胞的增殖活性明显大于WKY。当将G_1期间静态的星形胶质体暴露于胎儿血清血清时，在9-12H滞后期后观察到[^3H]胸苷的线性增加。尽管在三种菌株中的滞后周期的长度似乎并没有不同，但SHRSP细胞中胸苷的掺入速率大于WKY细胞中的胸苷。 SHRSR细胞显示SHRSP和WKY之间的中间掺入。在SHR和WKY的培养的血管平滑肌细胞中观察到了相似的结果。与WKY相比，SHRSP和SHRSR的总胆固醇水平显着降低。在三种菌株中比较了[^<14> c]乙酸或[^<14> c]甲酸[^<14> c]甲酸盐的胆固醇生物合成的活性。在SHRSP和SHRSR中，乙酸胆固醇的形成降低，但是在三种菌株中，甲瓦龙酸盐的胆固醇生物合成没有差异。 Alpha-Amanist提高了星形胶质细胞的α受体，并且细胞中的Ca^<++>水平增加。细胞Ca^<++>水平在三种菌株中治疗苯肾上腺素（10^<-5> -10^<-7> m）后，剂量依赖性升高，对SHR刺激的反应性高于WKY。这些异常在培养的血管平滑肌细胞中也指出，SHR可能具有相当一般的表型。