Hypertensive retinar vascular changes and hemorrhage in stroke-prone spontaneously hypertensive rats (SHRSP)

易发生中风的自发性高血压大鼠（SHRSP）的高血压视网膜血管变化和出血

• 批准号: 60570843
• 负责人: YOH Hamada
• 金额: $ 0.64万
• 依托单位: Kinki University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570843/
• 关键词: Hypertension; Hypertensive papilledema; Stroke; Stroke-prone sontaneously hypertensive rat; SHR; SHRSP; Trypsin-digested flat preparation; Fluorescein labelled dextrans; Laminin; Type 4 collagen; 乳頭浮腫

In stroke-prone spontaneously hypertensive rats (SHRSP), hypertensive vascular changes are observed near the optic disc and disc swelling frequently occurs after cerebral stroke. Pathological changes of retinal arterioles and capillaries in the optic disc andperipaplllary area of SHRSP were studied using the trypsin-digested flat preparation or the flat preparation of the retina following the injection of fluorescein labelled dextrans. Furthermore, to investigate the distribution of blood vessels in the retina and optic nerve of SHRSP, we stained the basement membrane of capillary endothelium by antibody against laminin and collagen type 4 using indirect immunofluorescent microscopy. By staining basement membrane components of the capillary endothelium, i.e. laminin and collagen type 4,it was possible to investigate the distribution of blood vessels at the retina and within the optic nerve. Hypertensivechanges of peripapillary vessels, retinal edema and the disc swelling were revealed by the funduscopy of SHRSP. Histologically, hypertensive arteriolar changes and capillary occlusions were observed in the peripapillary area. Hydropic changes were most severe in the prelaminar portion of the optic disc. In SHRSP with hypertensive papilledema, extravastion of fluoresceinlabelled dextrans was seen from the arteriole and the surrounding capillaries near the optic disc.

在自发性高血压大鼠（SHRSP）中，在视盘附近观察到高血压的血管变化，并且椎间盘溶解后经常发生大脑中风后。在注射荧光素标记的右旋胰蛋白酶后，使用胰蛋白酶消化的平坦制剂或视网膜的平坦制剂研究了视网膜和毛细血管的病理变化。此外，为了研究SHRSP的视网膜和视神经的血管的分布，我们使用间接免疫荧光显微镜染色了毛细血管内皮的基底膜和4型胶原蛋白。通过染色毛细血管内皮的地下膜成分，即层粘连蛋白和4型胶原蛋白，可以研究视网膜和视神经内血管的分布。 SHRSP的基础概念揭示了周围乳腺血管，视网膜水肿和椎间盘肿胀的高血压。在组织学上，在围绕乳腺癌区域观察到高血压小动脉的变化和毛细血管闭塞。在视盘的前期部分，水力发电的变化最为严重。在SHRSP中，有高血压乳头状果皮，从小动脉和周围的毛细管中可以看到荧光素的葡萄球菌的外部。

项目成果

浜田陽: Therapeutic Research. 5. 11-14 (1986)

Yo Hamada：治疗研究。5. 11-14 (1986)

Yoh Hamada: Journal of Hypertention. 4(Suppl.3). 417-419 (1986)

Yoh Hamada：高血压杂志。

Yoh Hamada: "Pathogenesis of hypertensive papilledema in SHRSP." Med. J. Kinki Univ.1. 10. 41-46 (1985)

Yoh Hamada：“SHRSP 中高血压视乳头水肿的发病机制。”

Yoh Hamada: "Fluorescein microscopic studies of retinal vessels near the optic disc in SHRSP." The Folia Ophthalmologica Japonica.36. 1392-1397 (1985)

Yoh Hamada：“SHRSP 视盘附近视网膜血管的荧光显微镜研究。”

Yoh Hamada: "Immunohistological studies on the distribution of basement membrane proteins, laminin and collagen type 4, in the retin and optic nerve of rat." Therapeutic Research.5. 11-14 (1986)

Yoh Hamada：“对大鼠视网膜和视神经中基底膜蛋白、层粘连蛋白和 4 型胶原蛋白分布的免疫组织学研究。”