Spectrophotometric Studies on the Primary Processes of Photosynthesis

光合作用初级过程的分光光度研究

基本信息

批准号：
01302064
负责人：
KATOH Sakae
金额：
$ 12.86万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01302064/
关键词：
Photosynthesis Primary Processes Spectrophotometry Chlorophyll Carotenoid Photosystem I Photosystem II Energy transfer 電子伝達光化学系I 光化学系II 蛍光光化学反応 EPR 時間分解分光測定

项目摘要

The Primary processes, i.e., energy transfer, photochemistry and electron transport of photosynthesis were investigated with a wide variety of spectrophotometric techniques. The materials used involved light-harvesting pigment-proteins and photochemically active preparations isolated from photosynthetic bacteria, cyanobacteria, algae and higher plants.P-700 was successfully reconstituted by adding chl a to chl-depleted PS I Particles and a structural role of antenna chl to stabilize PS I complexes was found for the first time (42). The differential absorption coefficient of P-700 was shown to be highly variable due to an electrochromic band shift of antenna chl a in detergent-treated PS I preparations (1,7). A stable Dl-D2-cyt. b-559 complex was prepared and the stoichiometry of subunit proteins and bound pigments were established (15).Binding of various synthetic quinones to quinone-binding sites of PS I (phylloquinone site) and PS II (*_B site) were investigated and structural parameters affecting the binding affinities of respective sites were determined (24, 25). A dark-stable S2 EPR signal and a new EPR signal derived from the S2 signal on illumination were found in inactivated oxygen evolving prepararions (17, 19). Efficiency of photoactivation of oxygen evolution was improved to a geat erxtent (21, 22) and His^+ was shown to serve as an oxidant of Mn during photoactivation (27). The emission spectrum of thermoluminescence was firstly determined (24, 25). Efficiency of energy transfer from carotenoids to bacteriochl was shown to decrease with increasing length and a distorted conformation of the conjugated double bond chains of carotenoids (54). Energy transfer in various photosynthetic organisms were intensively studied (64-72).

使用各种分光光度技术研究了光合作用的主要过程，即能量转移、光化学和电子传输。所使用的材料包括从光合细菌、蓝细菌、藻类和高等植物中分离的光捕获色素蛋白和光化学活性制剂。通过将叶绿素a添加到叶绿素耗尽的PS I颗粒中以及天线叶绿素的结构作用，成功地重构了P-700首次发现稳定 PS I 复合物 (42)。由于经洗涤剂处理的 PS I 制剂中天线叶绿体的电致变色带位移，P-700 的差异吸收系数表现出高度可变性 (1,7)。稳定的D1-D2-细胞色素。制备了 b-559 复合物，并建立了亚基蛋白和结合色素的化学计量 (15)。研究了各种合成醌与 PS I（叶绿醌位点）和 PS II（*_B 位点）的醌结合位点的结合，并确定了结构确定了影响各个位点的结合亲和力的参数 (24, 25)。在灭活的放氧制剂中发现了暗稳定的 S2 EPR 信号和源自照明时 S2 信号的新 EPR 信号 (17, 19)。光活化析氧效率大幅提高 (21, 22)，并且 His^+ 在光活化过程中可作为 Mn 的氧化剂 (27)。首先测定了热释光的发射光谱(24, 25)。从类胡萝卜素到细菌的能量转移效率随着类胡萝卜素共轭双键链长度的增加和构象的扭曲而降低 (54)。人们对各种光合生物的能量转移进行了深入研究(64-72)。