Spectrophotometric Studies on the Primary Processes of Photosynthesis

光合作用初级过程的分光光度研究

基本信息

批准号：
01302064
负责人：
KATOH Sakae
金额：
$ 12.86万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01302064/
关键词：
Photosynthesis Primary Processes Spectrophotometry Chlorophyll Carotenoid Photosystem I Photosystem II Energy transfer 電子伝達光化学系I 光化学系II 蛍光光化学反応 EPR 時間分解分光測定

项目摘要

The Primary processes, i.e., energy transfer, photochemistry and electron transport of photosynthesis were investigated with a wide variety of spectrophotometric techniques. The materials used involved light-harvesting pigment-proteins and photochemically active preparations isolated from photosynthetic bacteria, cyanobacteria, algae and higher plants.P-700 was successfully reconstituted by adding chl a to chl-depleted PS I Particles and a structural role of antenna chl to stabilize PS I complexes was found for the first time (42). The differential absorption coefficient of P-700 was shown to be highly variable due to an electrochromic band shift of antenna chl a in detergent-treated PS I preparations (1,7). A stable Dl-D2-cyt. b-559 complex was prepared and the stoichiometry of subunit proteins and bound pigments were established (15).Binding of various synthetic quinones to quinone-binding sites of PS I (phylloquinone site) and PS II (*_B site) were investigated and structural parameters affecting the binding affinities of respective sites were determined (24, 25). A dark-stable S2 EPR signal and a new EPR signal derived from the S2 signal on illumination were found in inactivated oxygen evolving prepararions (17, 19). Efficiency of photoactivation of oxygen evolution was improved to a geat erxtent (21, 22) and His^+ was shown to serve as an oxidant of Mn during photoactivation (27). The emission spectrum of thermoluminescence was firstly determined (24, 25). Efficiency of energy transfer from carotenoids to bacteriochl was shown to decrease with increasing length and a distorted conformation of the conjugated double bond chains of carotenoids (54). Energy transfer in various photosynthetic organisms were intensively studied (64-72).

使用多种分光光度计技术研究了光合作用的主要过程，即能量转移，光化学和电子传输。所使用的材料涉及从光合细菌，蓝细菌，藻类和较高植物中分离出的光化色素蛋白和光化活性制剂。P-700通过将CHL A添加到Chl depleded PS I颗粒中，成功地重构了CHL A，并在Antenna Chl的结构上稳定了Antenna Chl的结构，以稳定了Antenna Chl，以稳定稳定ps i confffs in Conffferes（第一个时间）（42）（42）。 P-700的差异吸收系数由于天线CHL A在洗涤剂处理的PS I制剂中（1,7）中天线CHL A的电染色带移位而显示出很高的变化（1,7）。稳定的DL-D2-Cyt。制备了B-559复合物，并建立了亚基蛋白和结合颜料的化学计量（15）。研究了各种合成奎因酮与PS I（Phylloquinone位点）和PS II（*_B位点）和PS II（*_B位点）的喹酮结合位点的结合，并研究了影响既定位点的结合位点（24）。在灭活的氧气进化准备中发现了一个深稳定的S2 EPR信号和从照明时S2信号得出的新的EPR信号（17、19）。将氧气演化的光活化效率提高到了Geat Erxtent（21，22），他的^+在光激活过程中被证明是Mn的氧化剂（27）。首先确定热发光的发射光谱（24，25）。从类胡萝卜素到细菌的能量转移效率随着长度的增加和类胡萝卜素的共轭双键链的变形而降低（54）。深入研究了各种光合生物的能量转移（64-72）。