Roles of the hypothalamic homeostatic system in the stress-induced brain-immune modulation.

下丘脑稳态系统在应激诱导的脑免疫调节中的作用。

• 批准号: 63440020
• 负责人: HORI Tetsuro
• 金额: $ 16.38万
• 依托单位: Kyushu University. (1989-1991)佐賀医科大学 (1988)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63440020/
• 关键词: Brain-Immune Interaction; Immune Cytokines; Interleukin-1; Interferon alpha; Brain Opioid System; Stress; Hypothalamic Neuron; Natural Killer Cells; CRF; 下垂体副腎皮質系; 脾臓交感神経; 発熱; 高体温; オピオイド・ペプチド; 視床下部; ナチュラルキラ-細胞; セロトニン; TNF; αMSH; オピエ-ト受容

We investigathed how the hypothalamus is involved in the reciprocal communication between the brain and the immune system thereby producing alterations in immunity, thermoregulation and feeding.(1) Effects of interleukin-1 (IL-1) and interferona (IFN alpha) on the hypothalamic neurons : . (a) Direct application of IL-1 beta (2.8-l2OpM) and IFNa (500-200OU/ml) altered the activities of thermosensitive and glucoseresponsive neurons in the hypothalamus of rats in such a way to produce fever and anorexia, respectively. While the actions of IL-1, 8 on hypothalamic neurons required the local production of PGE2, those of IFN a were mediated by the opioid receptors. The effects of IL-1, 8 on the neurons, like those on lymphocytes, were antagonized by a MSH. (b) Neurons in the organum vasculosum lamina terminalis (OVLT) were found to be more sensitive to PGE2 than those in the preoptic hypothalamus. Most OVLT warmsensitive neurons were inhibited by PGE2. The results provide further evidence for … More the critical involvement ofthe OVLT in mediating fever to blood-borne cytokines through the local release of PGE2.(2) Effects of temperatures, on the splenic NK cytotoxicity : Both the hyperthermic (39-49pﾟC) and hypothermic (34-36ﾟC) rats which were subjected to either environmental heat and cold stresses or hypothalamic cooling and warming exhibited the reduced NK activity. Preincubation at higher temperatures (39-40ﾟC) in vitro also decreased the NK activity. Thus, NK activity, unlike T cell functions, is depressed by febrile temperatures in vivo and in vitro.(3) Involvement of brain opioids and sympathetic nerves in the stress-induced immunosuppression : Intracerebral injections of IFN and 8 endorphin in the rat suppressed the splenic NK activity by activation of brain opioid receptors. This immunosuppression was found to be mediated predominantly by the splenic sympathetic nerve from the results of the experiments with recording of its electrical activity, its electrical stimulation, splenic denervation and splenic noradrenaline release as determined by in vivo microdialysis. Less

我们研究了如何参与大脑和免疫系统之间的倒数，从而产生了S免疫，温度调节和喂食。大鼠下丘脑中的葡萄糖神经因发烧和厌食的方式，而IL-1的作用对PGE2的局部产生，而IL-1的作用（ 1，8在神经元上，像淋巴细胞上的神经元一样，大多数OVLT的神经元被PGE2抑制。 PGE2的释放在较高的温度下（39-40°）在体外降低了NK活性，与T细胞功能不同，Vivo的温度与T细胞功能不同。抑制了脑蛋白阿片类受体的脾脏NK激活，从实验的结果中发现了脾脏的交感神经，其电刺激，脾脏刺激性的呼吸义和脾脏释放

项目成果

Kiyohara, T.: "Hypothalamic warm-sensitive neurons possess a tetrdotoxin-sensitive sodium channel with a high Q_<10>." Neurosci. Res.8. 48-53 (1990)

Kiyohara, T.：“下丘脑温敏感神经元拥有河豚毒素敏感钠通道，Q_<10> 较高。”

Hori, T.: "Effects of interleukin-1 and interferon-alpha on thermosensitive neurons and glucoreceptor neurons in the hypothalamus." J. Univ. Occup. Environ. Health. 11. 111-112 (1989)

Hori, T.：“白细胞介素 1 和干扰素 α 对下丘脑热敏神经元和葡萄糖感受器神经元的影响。”

HORI, T.: "Responses of anterior hypothalamic-preoptic thermosensitive neurons to locally applied capsaicin." Neuropharmacology. 27. 135-142 (1988)

HORI, T.：“下丘脑前部视前热敏神经元对局部应用辣椒素的反应。”

Shibata, M.: "Responses of anterior hypothalamic-preoptic thermosensitive neurons to substance P and capsaicin." Neuropharmacology. 27. 143-148 (1988)

Shibata, M.：“下丘脑前部视前热敏神经元对 P 物质和辣椒素的反应。”

Asami, T.: "Convergence of thermal signals on the reticulospinal neurons in the midbrain, pons and medulla oblongate." Brain Res. Bull.20. 581-596 (1988)

Asami, T.：“中脑、脑桥和延髓的网状脊髓神经元上的热信号会聚。”