Modulation of feeding behavior through brain histamine Hl-receptor

通过大脑组胺 Hl 受体调节进食行为

• 批准号: 62571025
• 负责人: KAZUMA Fujimoto
• 金额: $ 1.28万
• 依托单位: Faculty of Medicine, Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62571025/
• 关键词: Histamine H_1-receptor; Hypothalamus; Ventromedial Hypothalamus; Paraventricular Nucleus; Feeding Behavior; Neuronal Histamine; αーfluoromethylhistidine; ヒスタミンH_1受容体拮抗薬; 外側視床下野; 視床下部背内側核; 視索前野-前視床下野; H_1受容体; α-fluoromethylhistidine; ニューロン活動

To clarify the physiological role of hypotalamic histamine H_1-receptor in control of food intake, ingestive behavior and neuronal activities were investigated under blockade or diurnal flucturation of hypothalamic neuronal histamine. Histamine H_1-, but not H_2-receptor antagonists, potently induced feeding in a dose-related manner after infusion into rat third cerebroventricle in the light period. This feeding was elicited through ventromedial hypothlamus (VMH) and/or paraventricular neucleus (PVN). Elicitation was attenuated after infusion immediately before the dark when histamine content in the hypothalamus was low and abolished after intraperitoneal pretreatment with -fluoromethylhistidine (FMH), a specific suicide inhibitor of histidine decarboxylase. Electrophoretic application of a H_1-receptor antagonist to VMH neurons specifically suppressed activities of glucose-responding neurons. The suppressive effect was also attenuated by pretreatment with FMH. These results suggest that feeding induced by H_1-receptor antagonist is due to blokade of neuronal histamine at the site of histmaine H_1-receptors, at least in part, in VMH and PVN.

为了阐明下丘脑组胺H_1受体在控制食物摄入量中的生理作用，在封闭或下丘脑神经元组胺的昼夜波动下研究了摄取行为和神经元活性。组胺H_1-，而不是H_2受体拮抗剂，在光周期输注到大鼠第三个小脑后，以剂量相关的方式有效诱导喂养。这种喂养是通过腹侧下丘脑（VMH）和/或室室内的Neucleus（PVN）引起的。在黑暗之前，当下丘脑中的组胺含量低并用-Fluoromethylylylihypine（FMH）（一种特定的组氨酸脱羧酶自杀抑制剂）后，在黑暗前后的诱导减弱。 H_1受体拮抗剂对VMH神经元的电泳应用特异性抑制了葡萄糖反应神经元的活性。通过使用FMH预处理，抑制作用也会减弱。这些结果表明，H_1受体拮抗剂诱导的喂养是由于H_1受体受体部位的神经元组胺沟，至少部分在VMH和PVN中。

项目成果

T.Sakata: Experientia. 44. 216-217 (1988)

T.Sakata：体验。

藤本一眞: 臨床と研究. (1988)

藤本一马：临床实践和研究（1988）。

T.Sakata.: Brain Res.441. 403-407 (1988)

T.Sakata.：脑研究441。

Kazuma Fujimoto: European Journal of Pharmacology. (1988)

藤本一马：欧洲药理学杂志。

K.Fujimoto: Experientia.

K.Fujimoto：体验。