Study on growth and differentiation of human eosinophils

人嗜酸性粒细胞生长和分化的研究

基本信息

批准号：
62570532
负责人：
YOSHIE Osamu
金额：
$ 1.15万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

Human eosinophilic cell line EoL-1 was studied by using luminol-dependent chemiluminescence for respiratory burst upon stimulation with phorbol ester (TPA) and N-formylpeptide (fMLP). Treatment of EoL-1 with interferon- (IFN- ) for 5 days strongly enhanced TPA-inducible CL response. IFN- A had a similar effect. Tumor necrosis factor (TNF) also enhanced TPAinducible CL response of EOL-1 and, furthermore, was quite inhiditory to EoL-1 cell growth. On the other hand, interleukin 5 (IL-5) or granulocyte-macrophage colony stimulating factor (GM-CSF) had no augmenting effect on TPA-inducible CL in EoL-1. IL-5, however, slightly enhanced the effect of IFN- . Furthermore, both IL-5 and GM-CSF showed slight growth-promoting effects on EoL-1. EoL-1 pretreated with IFN- , - A or TNf also became capable of producing CL response to fMLP, and IFN- and TNF were again synergistic.EoL-1 treated with IFN- , - A, or TNF had abundant cytoplasm, but only TNF increased cells having distinct eosinophilic granules. IFN- enhanced this cytological effect of TNF. It was further demonstrated that the simultaneous treatment of EoL-1 with IFN- and TNF strongly increased the binding sites for phorbol diesters and also dramatically induced the surface expression of fMLP receptors. Taken together, EoL-1 seems to provide a valuable experimental model for the study of the ontogeny and the function of human eosinophils and as a source of their products.

通过使用鲁米诺依赖性化学发光来研究人嗜酸性细胞系 EoL-1 在佛波酯 (TPA) 和 N-甲酰肽 (fMLP) 刺激下的呼吸爆发。用干扰素 (IFN-) 处理 EoL-1 5 天，可强烈增强 TPA 诱导的 CL 反应。 IFN-A也有类似的作用。肿瘤坏死因子 (TNF) 还增强 EOL-1 的 TPA 诱导的 CL 反应，而且对 EoL-1 细胞的生长具有相当的抑制作用。另一方面，白介素 5 (IL-5) 或粒细胞巨噬细胞集落刺激因子 (GM-CSF) 对 EoL-1 中 TPA 诱导的 CL 没有增强作用。然而，IL-5 略微增强了 IFN-γ 的作用。此外，IL-5 和 GM-CSF 对 EoL-1 均表现出轻微的生长促进作用。用 IFN-、-A 或 TNF 预处理的 EoL-1 也能够对 fMLP 产生 CL 反应，并且 IFN- 和 TNF 再次具有协同作用。用 IFN-、-A 或 TNF 处理的 EoL-1 具有丰富的细胞质，但仅TNF增加的细胞具有明显的嗜酸性颗粒。 IFN-增强了TNF的这种细胞学效应。进一步证明，用 IFN-和 TNF 同时处理 EoL-1 强烈增加了佛波二酯的结合位点，并且还显着诱导了 fMLP 受体的表面表达。总而言之，EoL-1 似乎为研究人类嗜酸性粒细胞的个体发育和功能以及作为其产品的来源提供了一个有价值的实验模型。