PERSONAL IDENTIFICATION FROM MINUTE BLOODSTAINS BY DOT ELISA TECHNIQUES

通过点 ELISA 技术从微小血迹中进行个人身份识别

基本信息

批准号：
62570266
负责人：
ISHIZU Hideo
金额：
$ 1.09万
依托单位：
KOCHI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570266/
关键词：
Species identification Fetal blood identification Blood grouping Monoclonal antibody Dot blotting ELISA 血痕 α-フェトプロテイン IgA ドットイムノバインディングアッセイ

项目摘要

We have devised some methods using a dot ELISA technique to obtain-informations useful for the personal identification from bloodstains.1. In the identification of human bloodstains by dot ELISA methods using monoclonal antibodies against human serum proteins, the method using anti-human albumin (anit-ALB) was very sensitive and did not show cross-reaction with red-chested tamarin, one of new world monkeys, and non-primate animals. The method using anti-human immunoglobulin kappa chain (anti-Igk) was not so sensitive as the method using anti-ALB but the specificity was more improved and did not show any cross-reaction with primates other than chimpanzee. Our methods using anti-ALB and anti-Igk would be useful in forensic practice.2. For the discrimination between adult and fetal/neonatal bloodstains, we tried to detect IgA and AFP from the same bloodstain extract by the dot ELISA method using monoclonal antihuman IgA and anti-human AFP. By this method, we were able to discriminate fetal/neonatal bloodstains from adult ones until one year after the stain preparation. Using this method, bloodstains from pathologic adults with increased blood AFP level such as patients with hepatoma could be easily discriminated from fetal/neonatal bloodstains.3. ABO blood grouping from bloodstains was possible by the dot ELISA using monoclonal anti-A, Anti-B and anti-H, and the possibility of MN blood typing from bloodstains by the dot ELISA have been suggested.

我们已经使用DOT ELISA技术设计了一些方法，以获取对血迹的个人识别有用的信息1。在使用针对人血清蛋白的单克隆抗体来鉴定人类血迹时，使用抗人白蛋白（ANIT-ALB）的方法非常敏感，并且没有与重新抓龙蛋白酶的交叉反应，新世界猴子之一，以及非生物动物。使用抗人类免疫球蛋白kappa链（抗IGK）的方法不像使用抗alb的方法那样敏感，但是特异性得到了更高的改善，并且没有与黑猩猩以外的灵长类动物表现出任何交叉反应。我们使用抗Alb和anti-IGK的方法将在法医实践中有用2。对于成人和胎儿/新生儿血迹的区分，我们尝试使用单克隆抗抗人IGA和抗人AFP，通过DOT ELISA方法从相同的血迹提取物中检测出IgA和AFP。通过这种方法，我们能够将胎儿/新生儿血迹与成年血迹区分开，直到污渍制备后一年。使用这种方法，可以很容易地将血液AFP水平升高（例如肝癌患者）的病理性成年人与胎儿/新生儿血迹区分开。3。 DOT ELISA可能使用单克隆抗A，抗B和抗H可以从血迹中产生ABO血迹，并且已经提出了DOT ELISA从血迹中键入血迹的Mn血液的可能性。