STUDIES ON MUSCLE-ASSOCIATED SUBSTANCES FOR THE MEDICO-LEGAL DIAGNOSIS

用于法医诊断的肌肉相关物质的研究

基本信息

批准号：
10470119
负责人：
ISHIZU Hideo
金额：
$ 7.42万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

The contents of muscle-associated substances (MAS) in the forensic specimens were investigated. The content of cardiac troponin T (TnT) in myocardium was different among the investigated cases and had no correlation with the concentration of this protein in cardiac blood, as well as that of myoglobin (Mb). The contents of MAS of skeletal muscle were also different among cases and 19 sites investigated. Therefore the usefullness of the contents of MAS in muscle for medico-legal diagnosis in forensic autopsy could not be proved. Concentration of TnT and cardiac troponin I was higher in central/arterial blood than in peripheral/venous blood. CPK and creatine, that are more contained in skeletal muscle than in myocardium, were determined more in peripheral/venous blood than in central/arterial blood. Activities of GOT and GPT in the blood of vena cava inferior was higher than in blood obtained form any other site. These results may elucidate the state of postmortem circulation and diffusion of MAS or drugs in case of intoxication. When the MTAS concentration in blood or other body fluids was investigated from medico-legal aspects, the TnT and TnI concentrations in right and left heart blood were useful as a supplemental parameters to diagnose drowning. The creatinine concentration in pricardial fluid and the creatine concentrations in cerebrospinal fluid and vitreous humor were also useful for estimating postmortem interval. By detection of the human-specific sequence in the 3rd exon of Mb gene it was possibe to identify the human origin from forensic biological materials. The analysis of SNPs in the 2nd exon of Mb gene were useful for forensic personal identification and paternity test.

研究了法医标本中与肌肉相关物质（MAS）的含量。在被研究的病例中，心肌心肌中心脏肌钙蛋白T（TNT）的含量与心血中该蛋白质的浓度以及肌红蛋白（MB）的浓度无关。在调查的病例和19个地点中，骨骼肌Mas的内容也不同。因此，无法证明MAS在肌肉中对肌肉中的肌肉中的内容物在法医尸检中对医疗法律诊断的有用性。中央/动脉血液中TNT和心脏肌钙蛋白I的浓度高于外周/静脉血。与心肌相比，在骨骼肌中，CPK和肌酸在外周/静脉血液中的确定比中央/动脉血液中的确定更多。 vena cava劣等血液中GPT和GPT的活性高于获得其他部位的血液中的血液。这些结果可能会阐明死后循环的状态以及在中毒的情况下MAS或药物的扩散。当从药物法律方面研究血液或其他体液中的MTA浓度时，左右心血上的TNT和TNI浓度可作为补充参数可诊断溺水。脑脊液和玻璃体幽默中的肌酐浓度和肌酸浓度也可用于估计死亡后间隔。通过在MB基因的第三外显子中检测人类特异性序列，可以从法医生物学材料中识别人的起源。 MB基因第二外显子中SNP的分析对于法医的个人鉴定和亲子鉴定非常有用。