Ultrastrructural Variations of Gap Junctions Correlated with Tissue Types or Funtions.

间隙连接的超微结构变化与组织类型或功能相关。

基本信息

批准号：
61570012
负责人：
SHIBATA Yosaburo
金额：
$ 1.34万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

1. A simple new method of deep-etching, upside-down etching was developed in an attempt to obtain clearer images of replicas, especially of fresh untreated tissues. During deep-etching, specimens were kept downwards and shaken periodically so that non-volatile materials in the subliming ice could fall out of the frozen tissues. This improvemnet leads to a considerable reduction of sedimented debris of unetchable materials on the membrane surfaces of gap junction plaques.2. Our previous rapid-freeze, deep-etch replica studies have revealed the differences of cytoplasmic surfacesbetween heart and liver gap junction. Further studies were carried out to clarify these differences in various types of tissues.In surface mucous cells of stomach, absorptive epithelial cells of small and large intestine, and distal tubule cells of kidney, similar to the liver gap junctions, most of the cytoplasmic surface membranes were monparticulate. Whereas, in the cardiac atrium and ventricle, aortic endothe … More lium, and the ciliary process, cytoplasmic surface membrane of these non-epithelial gap junctions consistently revealed characteristic particulate patterns like a tiled surface made up of round tiles of vaious sizes. Close examinations disclosed that those granular materials were not merely attached to the membrane surface but protruded from the membrane interior as an integral component of gap junction particles. In the same replica area of pregnant rat uterus at labor, we found that surface membrane of myometrial smooth muscle gap junctions were particulate, but those of endometrial epithelium were smooth.3. The results of present study strongly suggest that cytoplasmic surface structures are different between epithelial and non-epithelial gap junctions; the non-epithelial gap junctions contain the extra cytoplasmic surface domain that is absent in the gap junctions of epithelial origin.It is interesting to note the similar distribution tendency of intermediate filament proteins between epithelial and non-epithelial tissues. Less

1。开发了一种简单的深蚀刻，倒置蚀刻的方法，以尝试获得更清晰的复制品图像，尤其是新鲜的未经处理的组织。在深蚀刻期间，将标本保持向下并定期摇动，以便升华的冰中的非挥发性材料可以从冷冻的组织中掉出来。这种改善网络导致考虑减少间隙连接斑块膜表面上不可药料的沉积碎片。2。我们以前的快速冻结，深蚀刻复制品研究表明，心脏和肝间隙连接之间的细胞质表面差异。进行了进一步的研究以阐明各种类型的组织中的这些差异。在插座的表面粘液细胞，小肠和大肠的吸收性上皮细胞以及肾脏的远端小管细胞类似于肝间隙连接，大多数细胞质表面膜都是蒙上了的。尽管在心脏中庭和心室中，主动脉内膜……更lium和纤毛过程，这些非上皮间隙连接的细胞质表面膜一致地揭示了特征颗粒模式，例如一个由vaious尺寸的圆形瓷砖组成的瓷砖表面。近距离检查表明，这些颗粒材料不仅连接到膜表面，而且是由膜内部产生的，作为间隙连接颗粒的组成部分。在分娩时怀孕大鼠子宫的相同复制区域中，我们发现肌层平滑肌间隙连接的表面膜是特别的，但是子宫内膜上皮的表面膜平滑。3。本研究的结果强烈表明，上皮和非上皮间隙连接之间的细胞质表面结构不同。非上皮间隙连接包含上皮起源间隙连接中不存在的额外细胞质表面结构域。有趣的是注意上皮和非上皮组织之间中间细丝蛋白的相似分布趋势。