Taxonomical and Ecological Studies on the Causative Dinoflagellates of Diarretic Shellfish Poisoning.

引起腹泻性贝类中毒的甲藻的分类学和生态学研究。

基本信息

批准号：
61560204
负责人：
ISHIMARU Takashi
金额：
$ 1.47万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560204/
关键词：
Diarretic Shellfish Poisoning (DSP)Dinoflagellate Taxonomy Culture ディノフィシス

项目摘要

Diarrehtic shellfish poisoning (DSP) is intoxification of shellfish which feed on dinoflagellates, Dinophysis spp.. DSP causes extensive impacts on fisheries industries in Japan as well as in European countries. As nobody have ever suceeded in culturing Dinophysis spp., physiological and ecological character of Dinophysis and the mechanism of the toxin production by the species are still unclear. Purposes of the present study are to make clear the nutitional style of Dinophysis and to establish culture method for them.At a first step, osmotrophic growth was tested. Cells of D. fortii were inoculated to enriched seawater media containing various organic compaunds. No growth were observed in axenic condition but some cells were survived in media contaminated with bacteria. Growth was also observed in seawater filterd by plankton net containing microflagellates. Thus, osmotrophy of D. fortii was denied but Phagotrophy was sugested. Then, Phagotrophic growth was tested by culturing D.fortii, D. acuminata and D. caudata with various species of micro algae as foods. As these Dinophysis species have orange fluorescence of phycobilin, cyanobacter or cryptomonad were thought to be contained in the cells of them. A cryptomonad, Plagioselmis sp. was observed to taken into the cells of 3 Dinophysis spp.. Growth of D. fortii and D. caudata were observed in culturing with Plagioselmis sp., but did not increase in large cell number. In order to establish cultures of Dinophysis spp., It is needed to study other factors which affect the growth of the species.

腹泻性贝类中毒 (DSP) 是指以甲藻、甲藻属为食的贝类中毒。DSP 对日本和欧洲国家的渔业造成广泛影响。由于尚未成功培养Dinophys spp.，Dinophys 的生理生态特性及其产生毒素的机制尚不清楚。本研究的目的是明确Dinophys的营养类型并建立其培养方法。第一步，进行渗透生长测试。将 D. fortii 细胞接种到含有各种有机化合物的富集海水培养基中。在无菌条件下没有观察到生长，但一些细胞在被细菌污染的培养基中存活。在经过含有微鞭毛虫的浮游生物网过滤的海水中也观察到了生长。因此，D. fortii 的渗透营养被否定，但吞噬营养被建议。然后，通过用各种微藻作为食物培养 D.fortii、D. acuminata 和 D. caudata 来测试吞噬生长。由于这些恐龙物种具有藻胆蛋白的橙色荧光，因此认为它们的细胞中含有蓝细菌或隐单胞菌。隐单胞菌，Plagioselmis sp。观察到Dinophys spp.被摄取到3个Dinophys spp.的细胞中。在与Plagioselmis sp.培养时观察到D. fortii和D. caudata的生长，但没有大量细胞数量增加。为了建立恐龙属的培养物，需要研究影响该物种生长的其他因素。