The functional role of photosynthesis-related genes in non-photosynthetic symbionts of corals

光合作用相关基因在珊瑚非光合作用共生体中的功能作用

• 批准号: 22KF0361
• 负责人: HUSNIK Filip
• 金额: $ 1.41万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KF0361/
• 关键词: Symbiosis; Microbiome; Evolution; Genomics; Ecology; Apicomplexa; Dinoflagellate; Coral

We collected coral colonies and performed DNA extraction. In order to identify apicomplexans associated with corals (order Corallicolida, also called corallicolids), PCR amplification was conducted using specific primers for the 18S rRNA region and Sanger sequencing. Preliminary results indicated that most corals from Japan and other parts of the Indo-Pacific Ocean associated with the same generalist corallicolid species. Still, some corals samples were associated with a distinct corallicolid lineage.Additionally, library preparation and MiSeq sequecing was used to evaluate the diversity of microbes associated with corals, using 16S rRNA (bacteria), 18S rRNA (Eukaryotes), and ITS2 rRNA (diniflagellates of the family Symbiodiniaceae) primers. We analyzed the data with bioinformatics following published pipelines. Preliminary results showed that corals associated with Symbiodiniaceae of the genera Cladocopium, Durisdinium and Symbiodinium. Moreover, corals from the Atlantic and the Indo-Pacific oceans have a different Symbiodiniaceae composition. In relation to bacteria, the mutualistic genus Endozoicomonas was the most common symbiont of corals. Some samples were associated with Vibrio spp., which are opportunistic pathogens. Samples from different regions on the Indo-Pacific Ocean analyzed so far had a different bacterial composition.

我们收集了珊瑚菌落并进行了DNA提取。为了鉴定与珊瑚相关的apicomplexans（也称为Corallicolids阶阶），使用18S rRNA区域和Sanger测序的特定引物进行了PCR扩增。初步结果表明，来自日本和印度太平洋其他地区的大多数珊瑚与同一经典性珊瑚糖类物种相关。尽管如此，使用16S rRNA（细菌），18S rRNA（eukaryotes）和is2 rrna（ies2 rrna symbibiiaciaceeaiaceeaiaceeaiac），使用了一些珊瑚样品与独特的珊瑚果实谱系有关。我们在发表的管道后用生物信息学分析了数据。初步结果表明，与氏菌，杜里斯丁岛和共生菌的共生性共生科相关的珊瑚。此外，来自大西洋和印度太平洋海洋的珊瑚具有不同的共生科组成。与细菌有关，互联属的依属植物是最常见的珊瑚共生体。一些样品与纤维状属属菌属相关，这些样本是机会性病原体。到目前为止，来自分析的印度太平洋海洋不同地区的样品具有不同的细菌组成。

项目成果

Global diversity of coral endosymbionts

珊瑚内共生体的全球多样性

• 发表时间: 2022
• 作者: Maria E. A. Santos;James D. Reimer;Masaru Mizuyama;Hiroki Kise;Wee H. Boo;Akira Iguchi;‘Ale’alani Dudoit;Robert Toonen;Marcelo V. Kitahara;Filip Husnik
• 通讯作者: Filip Husnik