NSF PRFB FY 2022: Identifying a Novel Green Fluorescent Small Molecule in a Heterotrophic Dinoflagellate

NSF PRFB 2022 财年：鉴定异养甲藻中的新型绿色荧光小分子

• 批准号: 2208914
• 负责人: Brittany Sprecher
• 金额: $ 13.8万
• 依托单位: Sprecher, Brittany
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2208914&HistoricalAwards=false
• 关键词: NSF; PRFB; FY; 2022; Identifying

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2022, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment, and Phenotypes. The fellowship supports research and training of the fellow that will contribute to the area of Rules of Life in innovative ways. Biofluorescence is a widespread light phenomenon where many organisms absorb and re-emit light at different wavelengths. The earliest investigations in to biofluorescence led to the discovery of the green fluorescence protein, GFP, from a jellyfish. This finding revolutionized biological science, leading to breakthroughs in marine, medical, and molecular sciences and ultimately resulting in a Nobel Prize. Further investigations into marine biofluorescence have led to discoveries of important functions in a number of organisms, and many of the discoveries from fluorescent research are being applied in medicine and biotechnology. Despite the importance of biofluorescence to the function and success of marine organisms, there are still many species where more research is needed to understand where, how, and why these organisms biofluoresce. This fellowship will investigate these questions while also providing a larger picture of the marine environment in which organisms that use biofluorescence live. The main objectives of the proposed research are to identify the structure, pathway, and environmental distribution of the heterotrophic dinoflagellate, Gyrodinium jinhaense AP17, novel green fluorescent molecule(s). Specifically, this fellowship aims to (I) determine G. jinhaense AP17’s fluorescent molecule’s structure(s), (II) identify the chemical and molecular pathways behind the green fluorescence, and (III) investigate the environmental distribution of this green fluorescence in both local and global contexts. These aims will be achieved through the integration of analytical chemical techniques, experimental treatments, transcriptomics, gene silencing, analysis of metatranscriptomic data, and environmental sampling. The fellow will gain new scientific skills in analytical chemistry as well as improve and advance current research skills. Moreover, the projects will include undergraduate researchers and at least one workshop where public participants will be introduced to dinoflagellates by sampling and observing them, with other plankton, under the microscope. All findings will be published and shared broadly from elementary classrooms to scientific meetings and with media to inspire interest in biology, oceanography, conservation, and the scientific process.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动为2022财年的生物学生物学研究金提供了一项NSF博士后研究奖学金，该研究研究了研究基因组，环境和表型之间相互作用的生活规则。奖学金支持对研究员的研究和培训，这些研究和培训将以创新的方式为生活规则做出贡献。生物荧光是一种广泛的光现象，许多生物在不同的波长下吸收并重新发射光。对生物荧光的最早研究导致从水母中发现了绿色荧光蛋白GFP。这一发现彻底改变了生物科学，导致海洋，医学和分子科学的突破，并最终导致诺贝尔奖。对海洋生物荧光的进一步研究导致了许多生物中重要功能的发现，并且在医学和生物技术中应用了许多荧光研究中的发现。尽管生物荧光对海洋生物的功能和成功至关重要，但仍有许多物种需要更多的研究来了解这些生物在何处，如何以及为什么生物荧光。该奖学金将调查这些问题，同时还提供了海洋环境的更大景象，其中使用生物荧光现场的生物。 The main objectives of the proposed research are to identify the structure, pathway, and environmental distribution of the heterotrophic dinoflagellate, Gyrodinium jinhaense AP17, novel green fluorescent molecule(s).Specifically, this fellowship aims to (I) determine G. jinhaense AP17’s fluorescent molecule’s structure(s), (II) identify the chemical and molecular pathways behind the green fluorescence, （iii）研究这种绿色荧光在本地和全球环境中的环境分布。这些目的是，研究员将获得分析化学方面的新科学技能，并提高并提高当前的研究技能。此外，这些项目将包括本科研究人员和至少一个研讨会，在该研讨会上，将通过在显微镜下与其他浮游生物一起采样和观察其他浮游生物，将公共参与者介绍给恐龙。所有发现将从小学教室到科学会议以及与媒体的广泛发表和共享，以激发人们对生物学，海洋学，保护和科学过程的兴趣。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子优点和更广泛影响的审查标准来评估来通过评估来获得的支持。