Interaction between nucleus and mitochondria in reconstituted cells.

重建细胞中细胞核和线粒体之间的相互作用。

• 批准号: 61540486
• 负责人: YOSHIDA Kazuo
• 金额: $ 1.28万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61540486/
• 关键词: mitochondria; nucleus; reconstituted cell; yeasts; stepwise transformation; genetics supporting system; 遺伝子解析支援コンピュータソフト

In order to study the interaction between nucleus and mitochondria in yeasts,we tried to develop in vitro cell system which reconstituted from anucleated cell, nucleus and mitochondria. For this purpose, we carried out the following investigations.1. Isolation of mutants which is suitable for cell reconstitution and transformation.2. Establishment of protplast conversion and its regeneration in giant yeasts,Wickerhamia fluorescene and Candida ingens.3. Development of versatile apparatus for pulsed field gel electrophoresis to separate large nuclear DNA, mtDNA and plasmid DNA. Determination of electrophoretic karyotypes of various yeasts by the device.4. Development of yeast genetic analysis programs (YGAP) for a microcomputer.5. Introduction of stepwise transformation which consists of transformation of conventional host cells and subsequent cytoductive transfer of vectors to destination cells with mitochondria by using kar1 mutation.6. Induction of heterothallic strains from homothallic strains by mutation and subseqent genetic analysis of mitochondria by mating.

为了研究核中核与线粒体之间的相互作用，我们试图开发从核细胞，核和线粒体重构的体外细胞系统。为此，我们进行了以下调查1。分离突变体，适合细胞重建和转化。2。在巨型酵母菌，Wickerhamia荧光和念珠菌Ingens中建立蛋白酶转化及其再生。3。开发用于脉冲场凝胶电泳的多功能设备，以分离大型核DNA，mtDNA和质粒DNA。测定设备的各种酵母的电泳核型。4。针对微型计算机的酵母遗传分析计划（YGAP）的开发5。引入逐步转化，包括传统宿主细胞的转化以及随后通过使用KAR1突变的线粒体向载体转移到目的地细胞的胞质转移。6。通过突变和通过交配对线粒体的亚分析分析从同质菌株中诱导异骨菌株。

项目成果

K.Yoshida;K.Suzuki: Yeast. 2. S378 (1986)

K.吉田；K.铃木：酵母。

K. Suzuki and K. Yoshida: "Stepwise Transformation in Saccharomyces cerevisiae Yeast." Yeast. 2. s378 (1986)

K. Suzuki 和 K. Yoshida：“酿酒酵母的逐步转化”。

K.Suzuki & K.Yoshida: Plant & Cell Physiol.27. 861-808 (1986)

铃木康

K.Yoshida: Yeast. 2. S438 (1986)

K.吉田：酵母。