Interaction between nucleus and mitochondria in reconstituted cells.

重建细胞中细胞核和线粒体之间的相互作用。

• 批准号: 61540486
• 负责人: YOSHIDA Kazuo
• 金额: $ 1.28万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61540486/
• 关键词: mitochondria; nucleus; reconstituted cell; yeasts; stepwise transformation; genetics supporting system; 遺伝子解析支援コンピュータソフト

In order to study the interaction between nucleus and mitochondria in yeasts,we tried to develop in vitro cell system which reconstituted from anucleated cell, nucleus and mitochondria. For this purpose, we carried out the following investigations.1. Isolation of mutants which is suitable for cell reconstitution and transformation.2. Establishment of protplast conversion and its regeneration in giant yeasts,Wickerhamia fluorescene and Candida ingens.3. Development of versatile apparatus for pulsed field gel electrophoresis to separate large nuclear DNA, mtDNA and plasmid DNA. Determination of electrophoretic karyotypes of various yeasts by the device.4. Development of yeast genetic analysis programs (YGAP) for a microcomputer.5. Introduction of stepwise transformation which consists of transformation of conventional host cells and subsequent cytoductive transfer of vectors to destination cells with mitochondria by using kar1 mutation.6. Induction of heterothallic strains from homothallic strains by mutation and subseqent genetic analysis of mitochondria by mating.

为了研究酵母细胞核和线粒体之间的相互作用，我们尝试开发由无核细胞、细胞核和线粒体重建的体外细胞系统。为此，我们进行了以下调查： 1．适合细胞重建和转化的突变体的分离。2．巨型酵母、荧光威氏酵母和假丝酵母原生质体转化及其再生的建立。 3．开发用于脉冲场凝胶电泳的多功能装置，用于分离大核 DNA、mtDNA 和质粒 DNA。利用该装置测定各种酵母菌的电泳核型。 4．微机酵母遗传分析程序（YGAP）的开发。 5．引入逐步转化，包括转化常规宿主细胞和随后使用kar1突变将载体细胞诱导转移至具有线粒体的目的细胞。6．通过突变从同宗菌株诱导异宗菌株，并通过交配对线粒体进行后续遗传分析。

项目成果

K.Yoshida;K.Suzuki: Yeast. 2. S378 (1986)

K.吉田；K.铃木：酵母。

K. Suzuki and K. Yoshida: "Stepwise Transformation in Saccharomyces cerevisiae Yeast." Yeast. 2. s378 (1986)

K. Suzuki 和 K. Yoshida：“酿酒酵母的逐步转化”。

K.Suzuki & K.Yoshida: Plant & Cell Physiol.27. 861-808 (1986)

铃木康

K.Yoshida: Yeast. 2. S438 (1986)

K.吉田：酵母。