Physio-chemical Analysis on the Mechanism of Conjugation between Eukyaryote and Prokaryote.

真核生物与原核生物接合机制的理化分析。

• 批准号: 04454015
• 负责人: YOSHIDA Kazuo
• 金额: $ 4.42万
• 依托单位: HOROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454015/
• 关键词: trans-kindom conjugation; organelles origin and evolution; conjugative plasmids; Escherichia coli; yeasts; YAC (yeast artificial chromosome); sex; endosymbiotic conjugation hypothesis; オルガネラの起源進化; 接合伝達; アグロバクリア; 真核微生物; 前核微生物; 凝集

Trans-kindom conjuation between prokaryotic Escherichia coli and eukaryotic Saccharomyces cerevisiae yeast required the sexual cell aggultination in which the conjugation occurred. The cell surface substances of both bacterium and yeast involved in the agglutination. The sex plasmid which were transmitted, were generally recirculized and replicated as plasmids in transconjugants. However, at very rare frequency, the plasmids were integrated into the transconjugants chromosomes by double crossing over. The conjugation process directly involves into the integration because this type integration is very rare in transformation. Newly constucted conjugative YACs in which oriT was inserted were successfully transferred into yeast recipient from E.coli. E.coli also could conjugate with Saccharomyces kluyveri which was a relatively remote species from S.cerevisiae. This conjugation induced a high frequent plasmid recombination. Agrobacterium tumafaciens could conjugate with S.cerevisiae. We compared T-DNA transfer with conjugate transfer of Ti-plasmid in Agrobacterium. Finally, we have proposed a novel hypothesis which describes the role of trans-kingdom conjugation on the origin and evolution of mitochondrion and chloroplast.

原核大肠杆菌和真核酿酒酵母之间的跨界接合需要发生接合的有性细胞凝集。细菌和酵母的细胞表面物质都参与凝集。传播的性质粒通常作为转接合子中的质粒进行再循环和复制。然而，质粒以非常罕见的频率通过双交换整合到转接合体染色体中。共轭过程直接涉及整合，因为这种类型的整合在转化中很少见。新构建的插入 oriT 的接合 YAC 已成功从大肠杆菌转移到酵母受体中。大肠杆菌还可以与克鲁维酵母（Saccharomyces kluyveri）结合，克鲁维酵母是距离酿酒酵母相对较远的物种。这种接合诱导了高频率的质粒重组。根癌农杆菌可以与酿酒酵母结合。我们比较了农杆菌中的 T-DNA 转移和 Ti 质粒的缀合物转移。最后，我们提出了一个新的假设，描述了跨界接合对线粒体和叶绿体的起源和进化的作用。

项目成果

吉田 和夫,大滝 保編著: "真核微生物の環境応答と遺伝子発現" 東北大学遺伝生態研究センター, 110 (1992)

Kazuo Yoshida、Tamotsu Otaki（编）：“真核微生物的环境响应和基因表达”东北大学遗传生态研究中心，110（1992）

Sekito, T., Okamoto, K., Kitano, H.and Yoshida, K.: "Yeast Hansenula wingei mitochondrial genome's complete DNA sequence demonstrated unique characteristics." Nucleic Acids Res. Symposium Series. 31. 233-234 (1994)

Sekito, T.、Okamoto, K.、Kitano, H. 和 Yoshida, K.：“酵母 Hansenula wingei 线粒体基因组的完整 DNA 序列表现出独特的特征。”

Sekito, T., Okamoto, Kitano, H.and Yoshida, K.: "The structure and organization of mitochondeial genome in Hansenula wingei.l" Complete DNA sequence and gene organization. XV International Botanical Congress. ABSTRACTS. 503 (1993)

Sekito, T.、Okamoto、Kitano, H. 和 Yoshida, K.：“Hansenula wingei.l 中线粒体基因组的结构和组织”完整的 DNA 序列和基因组织。

Inoue, T., Nishino, N., Kai, Y., Harada, S., Ohshiro, Y., Suzuki, S., Kohzuma, T., Shidara, S.and Iwasaki, H.: "Crystallization and preliminary X-ray studies on Pseudoazurin from Achromobacter cycloclasters IAM1013." J.Biochem.114. 761-762 (1993)

Inoue, T.、Nishino, N.、Kai, Y.、Harada, S.、Ohshiro, Y.、Suzuki, S.、Kohzuma, T.、Shidara, S. 和 Iwasaki, H.：“结晶和初步 X

T.Sekito,K.Okamoto,H.Kitano and K.Yoshida: "Yeast Hansenula wingei mitochondrial genome's complete DNA sequene demonstrated usique characteristics." Nucleic Acids Symposium Series. 31. 233-234 (1994)

T.Sekito、K.Okamoto、H.Kitano 和 K.Yoshida：“酵母 Hansenula wingei 线粒体基因组的完整 DNA 序列表现出独特的特征。”