A basic study on the development of diagnostic and treatment for periodontal diseases using monoclonal antibodies against human periodonopathic bacteria.

使用抗人牙周病细菌的单克隆抗体诊断和治疗牙周病的基础研究。

基本信息

批准号：
60570879
负责人：
NAITO Yuko
金额：
$ 1.15万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570879/
关键词：
IgG response B. gingivalis Adult periodontitis Monoclonal antibody Hemagglutinin LPS 診断 IgG抗体

项目摘要

Bacteroides gingivalis has been recognized as causing adult periodontitis. Antibody elevations in adult patients with periodontitis in response to the concentration of the organisms have been reported in our labolatory. The present research was done to determine which surface antigens was useful in detecting serum antibody to the microorganisms by immunoblotting analysis with monoclonal antibodies. Almost all sera obtained from the patients reacted with sonicate antigen, glass-beads extracted antige, and attachment factor(hemagglutinin) of the cells. Most sera from adult periodontitis sera reacted strongly with hemagglutinin, but a considerable variation in antibody levels against lipopolysaccharide(LPS) and capsular antigen was found. The specific IgG level to B. gingivalis was determined by a micro ELISA. A statistically positive correlation was found between IgG levels against the antigens and the degree of alveolar bone loss. On the other hand, no significant correlation was found between the antibody levels against the antigens and pocket depth. The sera obtained from patients reacted with hemagglutinin were confirmed by the immunoblotting tests. Sera from healthy individuals did not react with these antigens.It is interesting that most sera from patients with adult periodontitis possessed high antibody levels to hemagglutinin. This result indicates that addhessive surface structures such as hemagglutinin participate as a first step in B. gingivalis colonization and antigenic stimulation. The present findings emphasize the use of hemagglutinin to detect antibodies against B.gingivalis during the initial stages of adult periodontitis.

牙龈拟杆菌已被认为是导致成人牙周炎的原因。我们的实验室已报告了成年牙周炎患者的抗体随微生物浓度升高而升高的情况。本研究旨在通过单克隆抗体的免疫印迹分析来确定哪些表面抗原可用于检测微生物的血清抗体。几乎所有从患者获得的血清都与超声抗原、玻璃珠提取的抗原和细胞的附着因子（血凝素）发生反应。大多数成人牙周炎血清与血凝素反应强烈，但针对脂多糖（LPS）和荚膜抗原的抗体水平存在相当大的变化。通过微量 ELISA 测定牙龈芽孢杆菌的特异性 IgG 水平。研究发现，针对抗原的 IgG 水平与牙槽骨丢失程度之间存在统计学上的正相关关系。另一方面，针对抗原的抗体水平和袋深度之间没有发现显着相关性。从与血凝素发生反应的患者获得的血清通过免疫印迹测试得到证实。健康个体的血清不与这些抗原发生反应。有趣的是，大多数成人牙周炎患者的血清具有高水平的血凝素抗体。该结果表明，诸如血凝素之类的粘附表面结构参与了牙龈芽孢杆菌定植和抗原刺激的第一步。目前的研究结果强调在成人牙周炎的初始阶段使用血凝素来检测针对牙龈芽孢杆菌的抗体。