ISOLATION AND SOME PROPERTIES OF ADHERENT MATERIALS OF PORPHYROMONAS GINGIVALIS

牙龈卟啉单胞菌粘附物质的分离及其一些性质

基本信息

批准号：
05671518
负责人：
NAITO Yuko
金额：
$ 1.34万
依托单位：
KOKUSHIKAN UNIVERSITY (1994)Tokyo Dental College (1993)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671518/
关键词：
PERIODONTAL BACTERIA PORPHYROMONAS GINGIVALIS ADHERENCE VESICLE FIMBRIAE PELLICLE P.gingivalis コラーゲン

项目摘要

The adherence of Porphyromonas gingivalis to cell surfaces of periodontal tissues may play an important role in its pathogenicity. In this study, some selected strains of P.gingivalis, including both invasive and non invasive strains, were investigated for adherence to gingival ligament components. The test strains of P.gingivalis adhered to collagen, fibronectin and laminin to significantly different degrees. An overall positive correlation was noted between hydrophobicity and the number of cells that attached to collagen coated on hydroxyapatite beads. Fimbriae extracted from non invasive strains bound strongly to collagen, whereas invasive strains'fimbriae bound weakly to collagen or saliva coated hydroxyapatite.. We have also extracted to vesicle adhesin from Porphyromonas gingivalis and characterized. The adhesin of vesicle was extracted with the CHAPS,gel-filtration, and affinity with aruginine.Protein of 52 kDa was observed in SDS-PAGE,and indicated to hemagglutinin weakly, not trypsin-activity. These data suggest that 1) the test strains'fimbriae adhered to gingival tissue or pellicle to significantly different degrees ; 2) adhesin from vesicle was common to invasive and non invasive strains and was characterized to protein of 52kD ; 3) this adhesin has hemagglutinin weakly.

牙龈卟啉单胞菌对牙周组织细胞表面的粘附可能在其致病性中发挥重要作用。在这项研究中，研究了一些选定的牙龈卟啉单胞菌菌株（包括侵入性和非侵入性菌株）对牙龈韧带组件的粘附情况。牙龈卟啉单胞菌测试菌株对胶原蛋白、纤连蛋白和层粘连蛋白的粘附程度显着不同。疏水性与附着在羟基磷灰石珠上涂覆的胶原蛋白上的细胞数量之间存在总体正相关性。从非侵入性菌株中提取的菌毛与胶原蛋白强烈结合，而侵入性菌株的菌毛与胶原蛋白或唾液包被的羟基磷灰石微弱地结合。我们还从牙龈卟啉单胞菌中提取了囊泡粘附素并进行了表征。经CHAPS提取，凝胶过滤，与精氨酸亲和，SDS-PAGE检测到52 kDa的蛋白质，表明其具有微弱的血凝素活性，无胰蛋白酶活性。这些数据表明：1）试验菌株菌毛对牙龈组织或表膜的粘附程度存在显着差异； 2) 来自囊泡的粘附素对于侵袭性和非侵袭性菌株来说是常见的，并且被表征为52kD的蛋白质； 3)该粘附素具有弱血凝素作用。