SeaWall: Understanding environmentally induced cell wall remodelling in the green seaweed Ulva sp.

SeaWall：了解环境诱导的绿色海藻石莼属细胞壁重塑。

• 批准号: 520755331
• 负责人: Dr. Klaus Herburger, Ph.D.
• 金额: --
• 依托单位: Institut für Biowissenschaften (IfBi)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/520755331?language=en
• 关键词: SeaWall; Understanding; environmentally; induced; cell

Seaweeds such as the worldwide occurring green macroalgae Ulva sp. (Chlorophyta) face harsh environmental conditions in their coastal habitats, yet they produce tremendous biomass accumulations known as “green tides”. The bulk material in these blooms are multi-layered cell walls, which are a polysaccharide-rich matrix that surrounds every cell and constitute up to ~60% of the algal dry matter. Cell walls are the only physical barrier between the sensitive algal protoplast and the environment. This makes them key elements in stress survival, for example, by increasing the water holding capacity of cells or by assisting a controlled shrinkage and expansion during desiccation-rehydration cycles. However, it is largely unknown how the Ulva cell wall composition and architecture respond to external abiotic factors such as water scarcity. This is a fundamental gap in knowledge, because it limits our understanding of how seaweeds cope which stress in coastal habitats and sustain their high ecological significance. To better understand Ulva’s ecological success, we will combine eco-physiological monitoring with state-of-the-art bioimaging and chemical biology tools to investigate how environmental factors shape the composition, remodelling and architecture of the Ulva cell wall. First, we will collect various Ulva thalli from the field and establish stable cultures. Next, we will construct an environmental chamber allowing us to expose Ulva thalli to defined water, temperature, and light stresses typical for coastal habitats. Finally, we will implement imaging and chemical biology tools to visualize and quantify chemical and structural changes of the Ulva cell wall composition due to changing environmental conditions in vivo and in situ. We anticipate that our interdisciplinary approach will allow us to uncover unknown features of seaweed cell wall reorganisation (e.g. enzymatic remodelling of cell wall components), helping us to better understand how seaweeds cope with stress. This will provide crucial information for future seaweed farming efforts that aim to maximize biomass production and provide a foundation for unravelling the molecular basis of cell wall remodelling in seaweeds.

诸如全球发生的海藻发生了绿色大藻类ulva sp。 （绿藻）在其沿海栖息地面临危害环境条件，但它们产生了巨大的生物质积累，称为“绿色潮汐”。这些血液中的大量材料是多层细胞壁，它们是富含多糖的矩阵，周围每个细胞，占藻类干物质的约60％。细胞壁是敏感藻类原生质体和环境之间唯一的物理障碍。这使它们成为压力存活中的关键要素，例如，通过增加细胞的水位容量或在确定层流周期期间有助于受控的收缩和扩张。但是，在很大程度上，未知ULVA细胞壁组成和建筑如何应对外部非生物因素，例如水稀缺性。这是了解的根本差距，因为它限制了我们对海藻如何更好地了解Ulva的生态成功的理解，我们将结合生态生理学监测与最先进的生物成像和化学生物学工具，以研究环境因素如何塑造Ulva细胞壁的组成，重塑和建筑的环境因素。首先，我们将从该领域收集各种Ulva Thalli并建立稳定的文化。接下来，我们将建造一个环境室，使我们能够暴露乌尔瓦（Ulva）来定义沿海栖息地典型的水，温度和光压力。最后，我们将实施成像和化学生物学工具，以可视化和量化由于体内和原位环境条件变化而导致的ULVA细胞壁组成的化学和结构变化。我们预计我们的跨学科方法将使我们能够发现海藻细胞壁重组的未知特征（例如，细胞壁组件的酶促重塑），有助于我们更好地了解海藻如何应对压力。这将为未来的海藻种植工作提供关键信息，旨在最大化生物质生产，并为揭开海藻中细胞壁改造的分子基础提供基础。