Studies on the pathogenesis and treatment of immune disorders by the analysis of immunoreguratory mechanism.

通过分析免疫调节机制来研究免疫性疾病的发病机制和治疗。

• 批准号: 59440046
• 负责人: YATA Junichi
• 金额: $ 7.68万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59440046/
• 关键词: Immunodeficiency; Chronic thyroiditis; Myasthenia gravis; Autoantibody; Suppressor T cell; Hybridoma; 免疫調節; 自己抗体; 慢性甲状腺炎; 重症筋無力症; ヘルパーT細胞; T細胞抗原

In order to analyse the immunoreguratory system involved in the pathogenesis of immune disorders, human lymphocytes from normals, immunodeficiency patients and autoimmune diseases were studied in vitro.1. An experimental system to analyse antibody production from the lymphocytes stimulated by specific antigens such as ovalbumin,tetanus toxoid,thyroglobulin and acetylcholine-receptor was established.2. At least two distinct T cell subsets were proved to be involved for the regulation of antibody formation and both antigen specific and nonspecific suppressor factors were secreted from T cells.3. T cells from healthy individuals suppressed the autoantibody formation from the lymphocytes of chronic thyroiditis or myasthenia gravis.4. In stimulation with excess dose of thyroglobulin. T cells from healthy individuals secreted humoral factors suppressing anti-thyroglobulin antibody formation from the lymphocytes of chronic thyroiditis.5. Such T cells were fused with an established T cell leukemia cell line. Thus obtained hybridomas were cloned and one clone which secretes antigen specific suppressor humoral factor for anti-thyroglobulin antibody formation was obtained.6. Humoral factors which induce suppressor T cells were demonstrated in the sera from cancer patients. Alpha-fetoprotein also induced suppressor T cells.

为了分析与免疫疾病发病机理有关的免疫测量系统，在体外研究了来自正常患者，免疫缺陷患者和自身免疫性疾病的人淋巴细胞。1。一个实验系统，以分析由特异性抗原（例如卵蛋白，破伤风毒素，甲状腺球蛋白和乙酰胆碱受体）刺激的淋巴细胞产生的抗体产生。2。事实证明，至少有两个不同的T细胞子集与抗体形成有关，抗原特异性和非特异性抑制因子都从T细胞中分泌。3。来自健康个体的T细胞抑制了慢性甲状腺炎或肌无力重症的淋巴细胞的自身抗体形成。4。在刺激过多的甲状腺球蛋白中。来自健康个体的T细胞分泌的体液因子抑制了慢性甲状腺炎淋巴细胞的抗胸腺球蛋白抗体形成。5。此类T细胞与已建立的T细胞白血病细胞系融合。因此，将获得的杂交瘤克隆和一个克隆，该克隆分泌抗抗原特异性抑制因子，以形成抗蛋白质球蛋白抗体。6。癌症患者的血清中证明了诱导抑制T细胞的体液因子。 α-毒素还诱导抑制T细胞。

项目成果

Okawa,H.,Inada,M.,Suzuki,M. and Yata,J.: "Regulatory functions of neoplastic lymphoid cells and their humoral products." Cancer. 56. 309-317 (1985)

大川 H.、稻田 M.、铃木 M.

Okawa,H.;Takase,K.;Takagi,S.;Yata,J.;Matsumoto,M.;Matsumoto,T.: Cancer. 57. 732-736 (1986)

Okawa,H.；Takase,K.；Takagi,S.；Yata,J.；Matsumoto,M.；Matsumoto,T.：癌症。

Noma,T.;Yata,J.: J.Immunol.(1987)

Noma,T.；Yata,J.：《免疫学杂志》(1987)

Kawano,Y.,Noma,T. and Yata,J.: "Analysis of decreased autologous mixed lymphocyte reaction of cord blood lymphocytes." Asian Pascific J.Allerg.Immunol.2. 49-55 (1984)

河野，Y.，野间，T.

四宮範明,矢田純一: 臨床免疫. 17. 690-696 (1985)

Noriaki Shinomiya，Junichi Yada：临床免疫学 17. 690-696 (1985)。