Atomic force microscope and -spectroscope with bio-measurement head

带生物测量头的原子力显微镜和光谱仪

• 批准号: 519828155
• 金额: --
• 依托单位: Universität des Saarlandes
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/519828155?language=en
• 关键词: Atomic; force; microscope; spectroscope; bio

The applicants wish to apply for a latest generation atomic force microscope and spectroscope (hereafter abbreviated as AFM) with bio-measurement head to determine the initial adhesion processes of living microorganisms (bacteria, fungi, parasites) on clinically relevant, biotic and abiotic, surfaces. The new AFM, which is to be set up in a genetic engineering facility of safety level 2 (hereafter abbreviated as S2 GenTG laboratory), shall be used to record precise force-distance curves of pathogenic microorganisms of risk group 2 under conditions that come as close as possible to real infection events. Compared to our AFM currently in use (which is now 14 years old), the new instrument will have a significantly improved signal-to-noise ratio, faster imaging properties and a greatly extended z-range (possibility of controlled movement in the vertical axis). Our research will focus in particular on the interaction of microorganisms with implants commonly used in clinical practice such as long-term catheters, dental implants and stents (in combination with body fluids such as blood plasma or saliva, or implants explanted from patients) as well as with human single cells, cell cultures, organoids and tissue samples. Furthermore, we would like to use the new device to generate elasticity profiles of eukaryotic cells (cancer and immune cells) and tissue sections, to characterize membrane-to-cortex cross-linking of melanoma cells, and - for the first time - to image the surface topography of the adhesive organelle of the parasite Giardia duodenales by atomic force microscopy.

申请人希望申请最新一代带有生物测量头的原子力显微镜和光谱仪（以下简称AFM），以确定活体微生物（细菌、真菌、寄生虫）在临床相关的生物和非生物表面上的初始粘附过程。新的AFM设置在安全二级基因工程设施（以下简称S2 GenTG实验室）内，用于记录2级风险病原微生物在满足以下条件下的精确力-距离曲线：尽可能接近真实的感染事件。与我们目前使用的 AFM（已有 14 年历史）相比，新仪器将具有显着提高的信噪比、更快的成像特性和极大扩展的 z 范围（可以在垂直轴上进行受控运动） ）。我们的研究将特别关注微生物与临床实践中常用的植入物的相互作用，例如长期导管、牙科植入物和支架（与血浆或唾液等体液或从患者体内取出的植入物结合）与人类单细胞、细胞培养物、类器官和组织样本一样。此外，我们希望使用新设备生成真核细胞（癌症和免疫细胞）和组织切片的弹性分布，以表征黑色素瘤细胞的膜到皮质交联，并首次进行成像通过原子力显微镜观察寄生虫十二指肠贾第鞭毛虫粘附细胞器的表面形貌。