RADIOSENSITIVITY AND APOPTOSIS OF SPERMATOGONIA

精原细胞的放射敏感性和细胞凋亡

• 批准号: 09670917
• 负责人: HASEGAWA Masatoshi
• 金额: $ 0.32万
• 依托单位: GUNMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670917/
• 关键词: apoptosis; spermatogonia; radiosensitivity; p53; knockout mice; TUNEL; 精粗細胞; p53

The purpose of this study is to identify the mode of radiation-induced cell death in the tesitis and other tissue.Male C57BL or BALB/cA Jcl-nu mice were irradiated with single doses of 0.5, 2.5 or 5.0 Gy to investigate the morphological features of radiation-induced cell death. Male p53 knockout mice, homozygously null p53(-/-) and heterozygous p53(+I-) mice (CBA/OYC), and wild type mice (CBA/OYC) were irradiated with a single dose of 5.0 Gy to determine whether the radiation- induced cell death was p53-dependent.The radiation-induced cell death of spermatogonia reached a peak at 12 hours after irradiation, but that of other tissue was at 6 hours after irradiation. Nuclei of many abnormal spermatogonia were condensed and TUNEL-positive, though margination of nuclear chromatin and nuclear fragmentation was rare. The incidence of radiation-induced cell death in testis was markedly increased in p53(+I+) mice, but there was little change in p53(-/-) mice. Apoptosis of lymphocytes and fibrosarcoma cells inceased markedly following irradiation in the p53(+/+) or p53(+/-) genotype, but that increased just a little in the p53(-/-) genotype.Spermatogonial cell death induced by irradiation is compatible with p53-dependent apoptosis, though the morphological features are not always typical.

本研究的目的是确定睾炎和其他组织中放射诱导的细胞死亡的模式。对雄性C57BL或BALB/cA Jcl-nu小鼠进行0.5、2.5或5.0 Gy的单剂量照射，以研究其形态学特征。辐射引起的细胞死亡。雄性p53敲除小鼠、纯合p53(-/-)和杂合p53(+I-)小鼠(CBA/OYC)和野生型小鼠(CBA/OYC)用5.0 Gy的单剂量照射以确定是否辐射诱导的细胞死亡具有p53依赖性。精原细胞的辐射诱导细胞死亡在照射后12小时达到峰值，而其他组织的辐射诱导细胞死亡则为p53依赖性。照射后6小时。许多异常精原细胞的细胞核浓缩且呈 TUNEL 阳性，但核染色质边缘化和核碎片很少见。 p53(+I+)小鼠睾丸辐射诱导细胞死亡的发生率显着增加，但p53(-/-)小鼠变化不大。 p53(+/+)或p53(+/-)基因型受辐射后淋巴细胞和纤维肉瘤细胞凋亡显着增加，但p53(-/-)基因型仅略有增加。辐射诱导的精原细胞死亡是与 p53 依赖性细胞凋亡兼容，尽管形态特征并不总是典型的。

项目成果

Kazushige Hayakawa: "The prognostic significance of immunohistochemically detected p53 protein expression in non-small cell lung cancer treated with radiation therapy" Anticancer Res.18. 3685-3688 (1998)

Kazushige Hayakawa：“免疫组织化学检测 p53 蛋白表达在放射治疗的非小细胞肺癌中的预后意义”Anticancer Res.18。

Hasegawa M,Mitsuhashi N,Yamakawa M,Furuta M,Maebayashi K,Imai R,Hayakawa K,Niibe H.: "p53 protein expression and radiation-induced apoptosis in human tumors transplanted to nude mice" Radiat Med. 15. 171-176 (1997)

Hasekawa M、Mitsuhashi N、Yamakawa M、Furuta M、Maebayashi K、Imai R、Hayakawa K、Niibe H.：“移植到裸鼠的人类肿瘤中的 p53 蛋白表达和辐射诱导的细胞凋亡”Radiat Med。

Hideyuki Sakurai: "Early radiation effects in highly apoptotic murine lymphoma xenografts monitered by 31P magnetic resonance spectroscopy" Int.J.Radiat.Oncol.Biol.Phys.41. 1157-1162 (1998)

Hideyuki Sakurai：“通过 31P 磁共振波谱监测高度凋亡小鼠淋巴瘤异种移植物的早期辐射效应”Int.J.Radiat.Oncol.Biol.Phys.41。

Masatoshi Hasegawa: "Resistance of differentiating spermatogonia to radiation-induced apoptosis and loss in p53-deficient mice" Radiat.Res.149. 263-270 (1998)

Masatoshi Hasekawa：“p53 缺陷小鼠中分化精原细胞对辐射诱导的细胞凋亡和损失的抵抗”Radiat.Res.149。

Masatoshi Hasegawa: "P53 protein expression and radiation-induced apoptosis in human tumors transplanted to nude mice" Rediation Medicine. 15・3. 171-176 (1997)

Masatoshi Hasekawa：“移植到裸鼠的人类肿瘤中的 P53 蛋白表达和辐射诱导的细胞凋亡”Rediation Medicine 15・3（1997）。