Development of Novel Bio-Production and Separation Processes Based on Self-Organiizing Structure Formation

基于自组织结构形成的新型生物生产和分离工艺的开发

基本信息

批准号：
09650828
负责人：
KUBOI Ryoichi
金额：
$ 2.18万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

When cells and biological systems are exposed to lethal stresses, for example, heat stress, theyrespond to inducevarious latent functions, such as rapid synthesis of specific proteins including a set of heat shock proteins(HSPs), refolding of damaged proteins, translocation and release of proteins across membranes, formation of protein aggregates in an inert insoluble form etc.. These stress responsive or stimuli-responsive functions of cells, proteins, and membraneshave been quantitatively analyzed by the aqueous two-phase partitioning method etc. in the various levels and found to be triggered by the formation of the new transient structure between partly denaturedproteins and HSPs or cell membranes/liposomes, driven by the common principle of self-organIzation, and mediated by the transient hydrophobic Interaction among them. These functions have been successfully utilized for bio-production and bio-separation processes mainly integrating them with the stress induced self-assembled structure, such as stimuli-responsive polymers, aqueous two-phase systems (ATPS), and liposomes. When the cultivated cells or the protein mixture solution was exposed to the specific heat stress condition in the presence of liposomes, stimuli-responsive polymers and/or ATPS, the target protein could be trapped, refolded, translocated, and recovered selectively depending on the applied stress level and proteins.

当细胞和生物系统暴露于致命胁迫时，例如热应激，将其恢复到诱导性潜在功能，例如快速合成特定蛋白质，包括一组热休克蛋白（HSP）（HSP），重塑受损的蛋白质，蛋白质的易位，蛋白质的易位和蛋白质的释放，蛋白质的形式，蛋白质或反应型在质量构成的构造构成的构成剂量构成的构成剂构成骨质的固醇。通过水性两阶段分配方法等定量分析细胞，蛋白质和膜的功能。在各个级别中，被发现是由新的瞬态结构形成的一部分变性蛋白和HSP或细胞膜或细胞膜/脂质体之间的新瞬态结构触发的，并由普通的原理通过自我形成的互换来驱动。这些功能已成功用于生物生产和生物分离过程，主要将它们与应力诱导的自组装结构相结合，例如刺激反应性聚合物，水性两相系统（ATPS）和脂质体。当脂质体存在培养细胞或蛋白质混合溶液时，刺激反应性聚合物和/或ATPS暴露于特定的热应力状况时，可以根据所施加的应力水平和蛋白质选择性地将靶蛋白捕获，重新折叠，易位，并选择性地恢复。