Development of Separation and Purification Processes with Reverse Micelles Using Functional Reaction

利用功能反应开发反胶束分离和纯化工艺

基本信息

批准号：
63850189
负责人：
KUBOI Ryoichi
金额：
$ 2.3万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

1)Reverse Micelle Size Distribution and Mechanism of Protein Solubilization into Reverse Micelles The size and its distribution of reverse micelles prepared by dissolving Aerosol OT in isooctane were measured by using a dynamic light scattering method. These were independent of AOT concentration and were correlated with water content which was controlled by salt concentration in the bulk aqueous phase. A simple scheme for solubilization of proteins into micelle solution has been presented which assumes that the proteins adsorbed on the interface between aqueous and organic solutions may dissolve into the micelles when they encounter with the micelles of which sizes are greater than those of the proteins. The effect of the steric and electrostatic interaction between the micelles and proteins has also been taken into account.Separation of Lysozyme, alpha-Chymotrypsin and BSA was carried out as an application of the present solubilization scheme.2)Separation of Proteins with Reverse Mice … More llar Liquid Membranes Reverse micellar bulk liquid membrane was successfully applied to the separation of proteins with high selectivity by utilizing electrostatic interaction and/or steric interaction(size exclusion effect) between micelles and proteins. Highly selective uphill transport of proteins through the liquid membrane from an aqueous phase to another can be achieved by manipulating pH, concentration of KCl in both aqueous phases and initial water content in the membrane phase and also by selecting anionic or cationic surfactant( AOT or CTAB ). This is effected by the differences in the physical properties of the proteins to be separated such as isoelectric point, surface charge and molecular weight. Denaturation of proteins, mainly caused by complex formation with AOT molecules at low concentration of KCl in the aqueous phase for recovery, is considerably small compared with that encountered in conventional batch extraction. The liquid membrane separation system was further extended to the separation method utilizing the difference of transfer rate of proteins concerned. The combined liquid membrane system with both AOT and CTAB membranes is also effective for the simultaneous separation of proteins. Less

1）通过使用动态光散射方法测量，蛋白质溶解化为反向胶束的反向胶束分布和蛋白质溶解度的机制及其反向胶束的分布。这些是与AOT浓度无关的，并且与水含量相关，这是由大量水相中的盐浓度控制的。已经提出了将蛋白质溶液溶液溶液溶液中的简单方案，该方案假设在水性和有机溶液之间吸附的蛋白质时，当蛋白质与蛋白质大于蛋白质大的胶束时，它们可能会溶于胶束。还考虑了胶束和蛋白质之间的空间和静电相互作用的影响。分离溶菌酶，α-晶型胰蛋白酶和BSA的分离是作为本溶液方案的应用。2）蛋白质与蛋白质分离。蛋白质与反向小鼠的蛋白质分离……更多的液体液体液体含量较高的液体含量是较高的液体含量，较高的液态含量是较高的液体含量，远离型膜片，是依次的液体象征性的型号。利用胶束和蛋白质之间的静电相互作用和/或空间相互作用（尺寸排除效应）。可以通过操纵pH，水相中的KCL浓度以及在膜相中以及选择阴离子或阳离子表面（AOT或CTAB）来实现高度选择性的蛋白质通过液体从水相传输到另一个水相。这是由于要分离的蛋白质的物理特性的差异，例如等电点，表面电荷和分子量。与常规批次提取中遇到的蛋白质相比，蛋白质的变性主要是由水相中的AOT分子在水相中恢复的低浓度KCl的复合形成。利用相关蛋白质的转移速率差，将液体膜分离系统进一步扩展到分离方法。具有AOT和CTAB膜的液体膜系统的组合系统也可以有效地分离蛋白质。较少的