Development of Separation and Purification Processes with Reverse Micelles Using Functional Reaction

利用功能反应开发反胶束分离和纯化工艺

基本信息

批准号：
63850189
负责人：
KUBOI Ryoichi
金额：
$ 2.3万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

1)Reverse Micelle Size Distribution and Mechanism of Protein Solubilization into Reverse Micelles The size and its distribution of reverse micelles prepared by dissolving Aerosol OT in isooctane were measured by using a dynamic light scattering method. These were independent of AOT concentration and were correlated with water content which was controlled by salt concentration in the bulk aqueous phase. A simple scheme for solubilization of proteins into micelle solution has been presented which assumes that the proteins adsorbed on the interface between aqueous and organic solutions may dissolve into the micelles when they encounter with the micelles of which sizes are greater than those of the proteins. The effect of the steric and electrostatic interaction between the micelles and proteins has also been taken into account.Separation of Lysozyme, alpha-Chymotrypsin and BSA was carried out as an application of the present solubilization scheme.2)Separation of Proteins with Reverse Mice … More llar Liquid Membranes Reverse micellar bulk liquid membrane was successfully applied to the separation of proteins with high selectivity by utilizing electrostatic interaction and/or steric interaction(size exclusion effect) between micelles and proteins. Highly selective uphill transport of proteins through the liquid membrane from an aqueous phase to another can be achieved by manipulating pH, concentration of KCl in both aqueous phases and initial water content in the membrane phase and also by selecting anionic or cationic surfactant( AOT or CTAB ). This is effected by the differences in the physical properties of the proteins to be separated such as isoelectric point, surface charge and molecular weight. Denaturation of proteins, mainly caused by complex formation with AOT molecules at low concentration of KCl in the aqueous phase for recovery, is considerably small compared with that encountered in conventional batch extraction. The liquid membrane separation system was further extended to the separation method utilizing the difference of transfer rate of proteins concerned. The combined liquid membrane system with both AOT and CTAB membranes is also effective for the simultaneous separation of proteins. Less

1）反向胶束尺寸的分布和蛋白质的机制为反向胶束的大小和反向反向的分布，是在异辛烷中被溶解的反向胶质的分布，是AOT的浓度，并由核心含有核心，这是由散装水相中的盐收缩控制的水。 n呈现在水溶液和有机溶液之间的界面上的聚会可以溶解胶束的胶束，这些胶束的大小更大。 2）通过利用静电相互作用和/或空间相互作用n胶束和蛋白质高度选择性地上山蛋白质液体膜从另一个相位到另一个蛋白质到另一个蛋白质到另一个蛋白质，以高旋转性的含量撒在单独的蛋白上。在两个水相中的KCl可以在膜相位相位相位相位相位相位相相位相相位相相位相位相相位计相位相位相位相位相位相位相位相位相相位计的差异。蛋白质的重量。与在常规批处理中遇到的相比，在水相中，蛋白质与AOT AX的复合物形成相当小。 b膜也可有效地分离蛋白质