Mechanisms of the disassembly and reassembly of organelles during mitosis

有丝分裂过程中细胞器分解和重组的机制

• 批准号: 09480165
• 负责人: TAGAYA Mitsuo
• 金额: $ 8.45万
• 依托单位: Tokyo University of Pharmacy and Life Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480165/
• 关键词: endoplasmic reticulum; Golgi apparatus; mitosis; membrane fusion; NSF; muclear envelope; trimeric GTP-binding protein; VCP; 核膜; N-エチルマレイミド感受性因子

At the onset of mitosis vesicle-mediated protein transport is inhibited, and organelles such as the Golgi and nuclear envelope are disassembled into small vesicles. At telophase the small vesicles are fused each other to form intact organelles. Although recent studies revealed that NSF and VCP are involved in the fusion of Golgi-derived vesicles, the mechanisms of the assembly and disassembly of organelles have not been fully understood. In the present study we studied the mechanism of the biogenesis of organelles including the nuclear envelope, Golgi apparatus, and endoplasmic reticulum. The followings are the results obtained.1. NSF is associated with the membrane-embedded SNARE complex via SNAP.NSF is localized not only at the Golgi apparatus but also at the nuclear envelope. An antibody against a subunit of the SNARE complex, SNAP-25, inhibits the fusion of nuclear membrane vesicles in vitro, suggesting the involvement of the NSF-SNAP-SNARE complex in the nuclear membrane fusion.2. The Golgi apparatus is disassembled by nordihydroguaiaretic acid in a mechanism dependent on trimeric GTP-binding proteins. This disassembly was suppressed by overexpression of alpha_<12> or alpha_2, but not other species, suggesting the involvement of specific alpha subunits in the organization of this organelle.3. A novel syntaxin species (syntaxin 18) was identified. Syntaxin 18 is most likely a homologue of yeast Ufe1p, a protein involved in the fusion of nuclear or endoplasmic reticulum membranes. The role of syntaxin 18 in the organization of the endoplasmic reticulum is under investigation.

在有丝分裂囊泡介导的蛋白转运时，抑制了高尔基体和核包膜等细胞器中的细胞器中。在末期，小囊泡相互融合以形成完整的细胞器。尽管最近的研究表明，NSF和VCP参与了高尔基衍生囊泡的融合，但尚未完全了解细胞器的组装和拆卸机理。在本研究中，我们研究了细胞器生物发生的机制，包括核膜，高尔基体和内质网。以下是获得的结果。1。 NSF通过SNAP与膜上包裹的军鼓复合物相关。NSF不仅在高尔基体设备上，而且还位于核包膜上。 SNAP-25对SNAP-25亚基的一种抗体抑制了核膜囊泡体外的融合，这表明NSF-SNAP-SNARE复合物参与了核膜融合。2。高尔基体被北多糖酸的脱染色以一种取决于三聚体GTP结合蛋白的机制。这种拆卸被alpha_ <12>或alpha_2的过表达所抑制，而不是其他物种，这表明特定的α亚基参与了该细胞器的组织。3。鉴定出一种新型的索法蛋白种（语法18）。语法18很可能是酵母UFE1P的同源物，酵母UFE1p是一种参与核或内质网膜融合的蛋白质。索法蛋白18在内质网的组织中的作用正在研究中。

项目成果

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Mori 等人：“SecA 的氨基末端区域参与 SecG 将蛋白质易位到大肠杆菌膜囊泡中的功能。”

Tani et al.: "Hypothetical protein KIAA0079 is a mammalian homologue of yeast Sec24p." FEBS Letters in press. (in press).

Tani 等人：“假设蛋白 KIAA0079 是酵母 Sec24p 的哺乳动物同源物。”

Igarashi, M.et al.: "The soluble N-ethylmaleimide-sensitive factor attached protein receptor complex in growth cones : molecular aspects of the axon terminal development." J.Neurosci.17. 1460-1470 (1997)

Igarashi, M.等人：“生长锥中可溶性 N-乙基马来酰亚胺敏感因子附着蛋白受体复合物：轴突末端发育的分子方面。”

Takuma, et al.: "Evidence for the putative docking/fusion complex of exocytosis in parotid acinar cells." FEBS Lett.404. 34-36 (1997)

Takuma 等人：“腮腺腺泡细胞胞吐作用的假定对接/融合复合物的证据。”

Yamaguchi, T.et al.: "Possible involvement of heterotrimeric G proteins in the organization of the Golgi apparatus." J.Biol.Chem.272. 25260-25266 (1997)

Yamaguchi, T.et al.：“异源三聚体 G 蛋白可能参与高尔基体的组织。”