Mechanisms for assembly/disassembly of the nuclear envelope

核膜的组装/拆卸机制

基本信息

批准号：
10215205
负责人：
TAGAYA Mitsuo
金额：
$ 22.08万
依托单位：
Tokyo University of Pharmacy and Life Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10215205/
关键词：
nucleus mitosis membrane fusion syntaxin VCP NVL NSF SNAP SNARE SNAP-25

项目摘要

The nuclear envelope is disassembled at the onset of mitosis, dispersed throughout the cytoplasm, and is reassembled in daughter cells at telophase. Although a recent study has demonstrated that VCP participates in the assembly of the nuclear envelope, the function of VCP is not fully understood. The following progresses have been achieved this year.(i) Identification of the VCP-SVIP complex: VCP is a chaperone-like ATPase and, in cooperation with Ufd1p, participates in nuclear envelope assembly. We identified a new adaptor for VCP named SVIP (small VCP-interacting protein) that forms a complex distinct from the VCP-Ufd1p.(ii) Identification of syntaxin 18-binding proteins: Syntaxin 18 is located in the endoplasmic reticulum, which is contiguous with the outer nuclear membrane. We demonstrated that syntaxin 18 forms a complex with hZW10 and RINT-1, both of which are involved in cell cycle checkpoints. BNIP1, a pro-apoptotic protein, also associates with syntaxin 18. These results raise the possibility that syntaxin 18 complex plays a role not only in membrane fusion but also in cell cycle and apoptosis.(iii) Analysis of a nuclear VCP-like protein, NVL: The role of NVL, a nuclear VCP-like protein, remains unclear. We found that NVL is localized in nucleoli and interacts with an RNA helicase, Mtr4p. Co-expression of Mtr4p with NVL facilitated the nucleolar-localization of Mtr4p, suggesting that NVL is a chaperone-like protein for Mtr4p.

核包膜在有丝分裂的发作时被分解，分散在整个细胞质中，并在末期的子细胞中重新组装。尽管最近的一项研究表明，VCP参与核包膜的组装，但VCP的功能尚未完全了解。（i）VCP-SVIP复合体的识别：VCP是类似伴侣的ATPase，并且与UFD1P合作，参与了核包膜组件。我们确定了一个名为SVIP（小VCP相互作用蛋白）的VCP的新适配器，该适配器形成与VCP-UFD1P不同的复合物。（ii）鉴定语法素18结合蛋白：构成蛋白18位于内质网中，与外部核纤维膜相关。我们证明了Syntaxin 18与HZW10和RINT-1形成复合物，它们都参与细胞周期检查点。 BNIP1是一种促凋亡蛋白，也与索法蛋白18相关联。这些结果增加了索法蛋白18复合物不仅在膜融合中，而且在细胞周期和凋亡中都起作用。（iii）分析核VCP样蛋白，NVL的核VCP蛋白：NVL：NVL的作用：一种核VCP蛋白质的作用，仍然是核VCP类蛋白质，仍然是und-like tike-expield。我们发现NVL位于核仁中，并与RNA解旋酶MTR4P相互作用。 MTR4P与NVL的共表达促进了MTR4P的核仁核定化，这表明NVL是MTR4P的伴侣样蛋白。