Effects of mechanical stress and cytokines on articular chondrocytes from osteoporosis animals

机械应力和细胞因子对骨质疏松动物关节软骨细胞的影响

• 批准号: 08877303
• 负责人: KAKUTA Saburo
• 金额: $ 1.09万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Exploratory Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08877303/
• 关键词: OSTEOPOROSUS; MECHANICAL STRESS; CYTOKINE; ARTICULAR CHONDROCYTE; CELL DEATH

In order to clarify the mechanism of cause and development of osteoarthritis, the relation between mechanical stress and cytokines which play an important role for osteoporosis was investigated using a chondrocyte-like cell line established in our department.As the results, while it was evidenced that centrifugal force at 2000 rpm until 60 minutes accelated cell proliferation with time-dependency, excess force (3000rpm) induced cell death. 80 % of cells for 60 min.at 3000 rpm were dead. We already indicated that appoptosis of cells was induced by TNF-alpha considered as one of critical cytokine for post-osteomenoposal osteoporosis. Therefore the effects of TNF-alpha under mechanical stress was studied. It was showed that TNF-alpha was able to stimulate cell death under suitable mechanical stress (2000 rpm for 60 min.). These results indicated that the cells was not only injured by excess mechanical stress, but also injured by suitable stress, if TNF-alpha was containing there.

In order to clarify the mechanism of cause and development of osteoarthritis, the relation between mechanical stress and cytokines which play an important role for osteoporosis was investigated using a chondrocyte-like cell line established in our department.As the results, while it was evidenced that centrifugal force at 2000 rpm until 60 minutes accelated cell proliferation with time-dependency, excess force (3000rpm) induced cell 死亡。 80％的细胞为60分钟。在3000 rpm处死亡。我们已经表明，TNF-Alpha被认为是骨后骨骨质疏松症的关键细胞因子之一诱导细胞的蛋白质。因此，研究了TNF-α在机械应力下的作用。结果表明，TNF-Alpha能够在适当的机械应力下刺激细胞死亡（2000 rpm持续60分钟）。这些结果表明，如果TNF-Alpha含有TNF-Alpha，则细胞不仅因过量的机械应力受伤，而且还受到适当的应力伤害。