The research of pathogenesis, development's mechanism and treatment of Pars Tensa Cholesteatoma.

紧张部胆脂瘤的发病、发展机制及治疗研究。

• 批准号: 22591891
• 负责人: MORIYAMA Hiroshi
• 金额: $ 2.83万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591891/
• 关键词: 緊張部型真珠腫; 中耳粘膜再生; 遺伝子解析; 中耳腔ガス換気能; 術後成績 表皮増殖機序; 線維芽細胞; キャスペース 14; 表皮細胞; 粘膜再生; 増殖と分化; キャスペース14; 遺残真珠腫; ガス換気能; 術後成績; 表皮増殖機序; 鼻粘膜

Cartilage tympanoplasty for Pars tensa(PT) cholesteatoma prevent postoperative retraction ofn tympanic membrane in the long term, and it was found that the hearing improvement was good. There are some relations between Otisis media with effusion and PT-cholesteatoma. It was thought that fibroblasts in subepithelial layer were involved in activity of cholesteatoma epithelium and cholesteatoma extension. The interaction of epithelium and the subepithelial tissue play ley role for development of cholesteatoma. We cultured a rabbit nasal mucosa epithelial cells sheet in temperature-responsive culture plate by animal experiment, and we transplanted it to the middle ear, and good mucosa regenaration was confirmed. Regenerated mucosa shows almost normal conditions with a histologically and functionally(pressure measurement of the middle ear cavity). Weconfirm that Caspase-14 where 1.170 is associated with for the differentiation of epithelial cells specifically develops in cholesteatoma epithelial cells. It is suggested that Caspase-14 promoter helped treatment (disappearance of the persistentcholesteatoma epithelium) of the middle ear cholesteatoma.

软骨鼓室成形术治疗张力部胆脂瘤可长期防止术后鼓膜回缩，听力改善良好。中耳积液与PT-胆脂瘤有一定的关系。人们认为上皮下层成纤维细胞参与了胆脂瘤上皮的活性和胆脂瘤的扩展。上皮和上皮下组织的相互作用在胆脂瘤的发生发展中起重要作用。我们通过动物实验在温度敏感培养板上培养了兔鼻粘膜上皮细胞片，并将其移植到中耳，证实粘膜再生良好。再生的粘膜在组织学和功能上（中耳腔的压力测量）显示出几乎正常的状态。我们确认与上皮细胞分化相关的Caspase-14（1.170）在胆脂瘤上皮细胞中特异性发育。这表明Caspase-14启动子有助于中耳胆脂瘤的治疗（持续性胆脂瘤上皮的消失）。

项目成果

Identification of Specific Gene Expression Profiles in Middle Ear Cholesteatoma. Symposium-5 Genetic Aspect of Pathogenesis and Pathophysiology of Cholesteatoma

中耳胆脂瘤特异性基因表达谱的鉴定。

• 作者: 小荒井衛;岡谷隆基;中埜貴元;小松原琢;黒木貴一;Mamoru Yoshikawa
• 通讯作者: Mamoru Yoshikawa

癒着性病変に対するcartilage tympanoplastyの術後成績

软骨鼓室成形术治疗粘连病变的术后效果

• 发表时间: 2010
• 作者: 田中康広;小島博己;穐吉亮平;小森学;山本和央;森山寛
• 通讯作者: 森山寛

自己由来粘膜再生シートを用いた鼓室形成術に対する再生医療の応用-ヒト鼻粘膜を用いた臨床応用に向けて-

再生医学在自体粘膜再生片鼓室成形术中的应用 - 人鼻粘膜的临床应用 -

• 发表时间: 2010
• 作者: 濱孝憲;山本和央;穐吉亮平;田中康広;小島博己;森山寛
• 通讯作者: 森山寛

家兎を用いた鼻粘膜上皮細胞シート移植による中耳粘膜再生-小動物CTによる中耳含気腔の検討-

家兔鼻粘膜上皮细胞片移植再生中耳粘膜再生-小动物CT检查中耳气腔-

• 发表时间: 2010
• 作者: 中埜貴元;小荒井衛;乙井康成;小林知勝;櫻井武司;山本和央
• 通讯作者: 山本和央

幼児滲出性中耳炎のチューブ抜去時蜂巣発育度と予後との関係

小儿渗出性中耳炎拔管时蜂窝状发育程度与预后的关系

• 发表时间: 2012
• 作者: 山本和央;小島博己;谷口雄一郎;森山 寛;山本耕司，内水浩貴，近藤悠子，森山 寛
• 通讯作者: 山本耕司，内水浩貴，近藤悠子，森山 寛