Molecular mechanism of genetic DNA rearrangements in fission yeast

裂殖酵母遗传DNA重排的分子机制

• 批准号: 21247027
• 负责人: IWASAKI Hiroshi
• 金额: $ 29.29万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21247027/
• 关键词: 相同組換え; DNA二重鎖切断; DNA修復; DNA鎖交換反応; 分裂酵母; Rad22; MRN複合体; Ctp1; Dmc1

We are very much interested in molecular mechanism of genetic DNA rearrangements(e. g. homologous recombination), and thus during this proposed research period we tried to elucidate some functions of proteins involved in genetic rearrangements in fission yeast, which was used as a model biological system. Among several approaches we took for this aim, we had a great achievement for analysis of Dmc1 recombinase that works for meiotic recombination. We demonstrated fission yeast Dmc1-mediated Holliday junction formation in vitro. The directionality is 5'-3', which is the same as that by bacterial RecA but the opposite by Rad51 recombinases. Our finding is very important to understand for molecular mechanisms of meiotic recombination that accompanies high level of crossover products.

我们对遗传DNA重排（例如同源重组）的分子机制非常感兴趣，因此在本研究期间，我们试图阐明裂殖酵母中参与遗传重排的蛋白质的一些功能，该酵母被用作模型生物系统。在我们为此目的采取的多种方法中，我们在分析适用于减数分裂重组的 Dmc1 重组酶方面取得了巨大成就。我们在体外证明了裂殖酵母 Dmc1 介导的霍利迪连接体形成。方向性为5'-3'，与细菌RecA的方向相同，而Rad51重组酶的方向相反。我们的发现对于理解伴随高水平交叉产物的减数分裂重组的分子机制非常重要。

项目成果

Molecular mechanism of DNA strand exchange reaction, a central reaction of homologous recombination mediated by recombinases

DNA链交换反应的分子机制，这是重组酶介导的同源重组的核心反应

• 发表时间: 2011
• 作者: 池田幸樹;八木美江子;森田鉄兵;金井昭夫;饗場弘二;Iwasaki H.
• 通讯作者: Iwasaki H.

Regulation of strand exchange reactions by mediator proteins

介导蛋白对链交换反应的调节

• 发表时间: 2009
• 作者: 松永藤彦;石野良純;Hiroshi Iwasaki;M. Hayashi;小林武彦;Hiroshi Iwasaki
• 通讯作者: Hiroshi Iwasaki

Human CtIP and its homolog : Team players in DSB resection games. In DNA repair. On the pathways to Fixing DNA Damage and Errors

人类 CtIP 及其同源物：DSB 切除游戏中的团队成员。

• 发表时间: 2011
• 作者: Tsutsui Y;Kawasaki A and Iwasaki H.
• 通讯作者: Kawasaki A and Iwasaki H.

An in vitro assay for monitoring the formation and branch migration of Holliday junctions mediated by a eukaryotic recombinase

监测真核重组酶介导的霍利迪连接体形成和分支迁移的体外测定

• 发表时间: 2011
• 期刊: Methods Mol. Biol
• 作者: 大泉嶺;高田壮則;Murayama Y and Iwasaki H
• 通讯作者: Murayama Y and Iwasaki H

Purification and crystallization of Swi5 and Swi5-Sfr1 complex from fission yeast

裂殖酵母中 Swi5 和 Swi5-Sfr1 复合物的纯化和结晶

• 发表时间: 2010
• 期刊: Acta Crystallogr Sect F Struct Biol Cryst Commun
• 作者: Kuwabara N;Hashimoto H;Yamada N;Unzai S;Ikeguchi M;Sato M;Murayama Y;Iwasaki H and Shimizu T
• 通讯作者: Iwasaki H and Shimizu T