Analysis of antigen-uptake network at mucosal epithelial layer

粘膜上皮层抗原摄取网络分析

• 批准号: 20249028
• 负责人: KIYONO Hiroshi
• 金额: $ 32.53万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20249028/
• 关键词: 粘膜免役; M細胞; 上皮細胞; 抗原取り込み; 共生細菌; 粘膜免疫; UEA-1; フコシル化

M (Microfold) cells and related subpopulation cells are known as specialized cells for antigen uptake. To reveal the extracellular regulating factors and the mechanisms for antigen uptake and their differentiation of M cells, we identified several M cell-specific genes, and investigated there functions on M cells. As a result, it was suggested that inducible M-like cells, which have been discovered by our group, were an intermediate cells between Peyer's patch M cells and intestinal epithelial cells. We succeeded in identifying GP2 and MLP as novel M cell-specific molecule. In the case of GP2, our collaborative efforts revealed that GP2 could capture some bacterial groups via FimH protein, and acted as a scaffold to uptake them efficiently. In addition, we established monoclonal anti-GP2 antibody, and verified that this antibody was applicable for effective vaccine antigen delivering to M cells.

M（微折叠）细胞和相关亚群细胞被称为抗原摄取的专门细胞。为了揭示M细胞的细胞外调节因子以及抗原摄取和分化的机制，我们鉴定了几个M细胞特异性基因，并研究了它们在M细胞中的功能。结果表明，本课题组发现的诱导型M样细胞是介于派尔氏淋巴结M细胞和肠上皮细胞之间的中间细胞。我们成功地将 GP2 和 MLP 鉴定为新型 M 细胞特异性分子。就 GP2 而言，我们的合作表明 GP2 可以通过 FimH 蛋白捕获一些细菌群，并充当有效吸收它们的支架。此外，我们还建立了抗GP2单克隆抗体，并验证了该抗体适用于将疫苗抗原有效递送至M细胞。

项目成果

Mucosal regulatory cells in the gastrointestinal tract and periodontium.

• DOI: 10.1111/j.1600-0757.2009.00335.x
• 发表时间: 2010-10
• 期刊: Periodontology 2000
• 影响因子: 18.6
• 作者: I. Takahashi;K. Fujihashi;H. Kiyono

Basic principals of immune responces : innate versus adaptive immunity

免疫反应的基本原理：先天免疫与适应性免疫

• 发表时间: 2010
• 作者: Sakuma M;Morimoto T;Kubota Y;Seki S;Takada K;Hiraide A;HIROSHI KIYONO
• 通讯作者: HIROSHI KIYONO

Sphingosine 1-phosphate-dependent trafficking of peritoneal B cells requires functional NFkB-inducing kinase in stromal cells.

腹膜 B 细胞的鞘氨醇 1-磷酸依赖性运输需要基质细胞中功能性 NFkB 诱导激酶。

• 发表时间: 2008
• 期刊: Blood. 111
• 作者: Kunisawa;J.;Gohda;M.;Kurashima;Y.;Ishikawa;I.;Higuchi;M . and Kiyono;H.
• 通讯作者: H.

Mucosal chaperoning nanogel vaccine for the induction of protective immunity.

用于诱导保护性免疫的粘膜陪伴纳米凝胶疫苗。

• 发表时间: 2010
• 作者: KIYONO H.

Rice-Based Vaccine : New Generation of Mucosal Vacine

大米疫苗：新一代粘膜疫苗

• 发表时间: 2009
• 作者: Hiroyuki Kusuhara;Yuichi Sugiyama;清野宏
• 通讯作者: 清野宏