Characterization of Oriented Thin Films of DNA

DNA 定向薄膜的表征

• 批准号: 13132205
• 负责人: OKAHATA Yoshio
• 金额: $ 31.74万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13132205/
• 关键词: DNA; Electro conductivity; Orientation; Electro luminescent; One molecular observation; Fluorescence Correlation Snectrosco; 蛍光相関分光法; DNAフィルム; 伝導小生; 配向化DNA; DNA・脂質複合体; LB膜; DNAポリメラーゼ; 水晶発振子; DNA固定化発振子; 4穴式水晶発振子; 酸素反応機構; 脂質コンプレックス; 電気伝導度

Deoxyribonucleic acids (DNAs) are important as a source of biological information depending on their base sequences. DNA is also interesting as a molecular material that shows a long rod-like duplex structure with base-pair stacking-the base separation is 3.4 Å while the diameter of the duplex is about 20 Å, therefore appears to be a good candidate for one-dimensional energy transfer and conduction along the p electron clouds of the stacked bases.In this report, we summarize preparations of DNA-aligned Langmuir-Blodgett (LB) film and its anisotropic electron conductivity.The DNA aligned film was prepared as follows. On the aqueous solution of DNA from Salmon testes and intercalator (ethidium bromide), Langmuir monolayer of cationic lipids were prepared, and DNA-lipid complexes were transferred on a substrate. It was confirmed by polarized absorption spectra that DNA strands aligned along a dipping direction.The dc conductivity was measured by using an ammeter in atmosphere. When the DNA film was put on the comb-shaped electrode as DNA strands aligned perpendicularly to two Au electrodes, a large ohmic current was observed that increased linearly with increasing the applied voltage up to 0.1 V. On the contrary, when the film was put as DNA strands aligned parallelly to two electrodes, electric current was hardly observed even at the voltage of 0.1 v.

脱氧核糖核酸（DNA）作为生物学信息的来源很重要，具体取决于它们的碱基序列。 DNA作为一种分子材料也很有趣，它显示出长杆状的双工结构，具有碱基的堆叠 - 基本分离为3.4Å，而双工的直径约为20Å，因此似乎是沿堆栈基本的PELECTER cOLLAD的一维能量转移和抑制的良好候选者，我们在此报告中，我们的准备工作，我们的准备工作，我们的准备工作。膜及其各向异性电子电导率。DNA排列的膜如下制备。在鲑鱼测试和插盐剂（溴化乙锭）的DNA水溶液上，制备了阳离子脂质的Langmuir单层，并在底物上传递DNA-脂质复合物。通过极化抽象光谱证实，沿浸入方向对齐的DNA链。直流电导率是通过在大气中使用仪表表测量的。当将DNA膜放在组合形的电极上时，DNA链垂直于两个Au电极对齐时，观察到一个较大的欧姆电流，随着将施加的电压增加到0.1 V的增加，线性增加了。在对比度上，当膜被作为DNA作为DNA作为DNA等于两个电子电流时，偶尔的VOL偶数VOL偶然地对比。

项目成果

Kinetic Studies of Amylopectin Cleavage Reactions Catalyzed by Phosphorylase b using a 27 MHz Quartz Crystal Microbalance

使用 27 MHz 石英晶体微天平研究磷酸化酶 b 催化的支链裂解反应的动力学

• 发表时间: 2004
• 期刊: J.Am.Chem.Soc. 126
• 作者: H.Nishino;A.Murakawa;T.Mori;Y.Okahata
• 通讯作者: Y.Okahata

H.Furusawa, A.Murakawa, S.Fukusho, Y.Okahata: "In vitro Selection of N Peptide-Binding RNA on a Quartz-crystal Microbalance to Study a Sequence-Specific Interaction of Peptide and Loop RNA"ChemBioChem. 217-220 (2003)

H.Furusawa、A.Murakawa、S.Fukusho、Y.Okahata：“在石英晶体微天平上体外选择 N 肽结合 RNA 以研究肽和环 RNA 的序列特异性相互作用”ChemBioChem。

川崎 剛美, 岡畑 恵雄: "サケ精子のDNAから作るバイオフィルム"未来材料. 3. 46-51 (2003)

Takemi Kawasaki，Keio Okahata：“由鲑鱼精子 DNA 制成的生物膜”Future Materials。3. 46-51 (2003)。

H.Matsuno, Y.Okahata: "Direct Monitoring of DNA Cleavages catalyzed by an ATP-dependent Deoxyribonuclease on a 27 MHz Quartz-Crystal Microbalance"Chem.Commun. 470-471 (2002)

H.Matsuno、Y.Okahata：“在 27 MHz 石英晶体微天平上直接监测 ATP 依赖性脱氧核糖核酸酶催化的 DNA 裂解”Chem.Commun。

Fabrication of Metal Nanowires by Electroless Plating of DNA

DNA 化学镀制备金属纳米线

• 发表时间: 2005
• 期刊: e-J.Surf.Sci.Nanotech 3
• 作者: Y.Imai;M.Ishida;M.Kakimoto;K.Ijiro et al.
• 通讯作者: K.Ijiro et al.