Development of 96-type and multi-type quartz-crystal microbalance

96型及多型石英晶体微天平的研制

• 批准号: 13558079
• 负责人: OKAHATA Yoshio
• 金额: $ 8.64万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13558079/
• 关键词: DNA hybridization; quartz-crystal microbalance; multi-sensor; 4-hole type QCM; QCM; DNA; たんぱく質

The purpose of this research is to develop a multi-type (96 hole)quartz-crystal microbalance (QCM In this paper, we introduce a 27-MHz quartz-crystal microbalance (QCM) as a new tool to detect DNA-protein/peptide interactions in an aqueous buffer solution. From the time course of the frequency decrease (mass increase), the binding amount (Δm), association constant (Ka), binding and dissociation rate constant (k1 and k1) can be obtained. When dsDNA was immobilized on a QCM, binding kinetics of antibiotics, a Zinc-finger type peptide, a restriction enzyme, and a polymerase to DNA strands could be obtained in aqueous buffer solution. The 27-MHz QCM is highly sensitive and quantitative enough to detect various molecular interactions on DNA double strands.

这项研究的目的是开发多型（96个孔）石英 - 晶体微生物（QCM本文，我们介绍27-MHz Quartz-cyartz-crystal微量平衡（QCM）作为一种新工具，作为检测DNA-蛋白/肽相互作用的频率降低的频率（频率）（频率）的新工具。当将dsDNA固定在QCM上，抗生素的结合动力学，锌指型肽，限制性酶，以及在DNA链条上的多个态度，可以在QCM上固定（Ka），结合和解离速率常数（K1和K1）。 DNA双链上的分子相互作用。

项目成果

H.Furusawa, Y.Kitamura, N.Hagiwara, T.Turimoto, Y.Okahata: "Binding Kinetics of the Toroidal-Shaped PCNA to DNA Strands on a 27 MHz Quartz-Crystal Microbalance"ChemPhysChem.. No.5. 446-448 (2002)

H.Furusawa、Y.Kitamura、N.Hagiwara、T.Turimoto、Y.Okahata：“27 MHz 石英晶体微天平上环形 PCNA 与 DNA 链的结合动力学”ChemPhysChem.. No.5。

M.Tanaka, A.Mochizuki, T.Motomura, K.Shimura, M.Onishi, Y.Okahata: "In Situ Studies on Protein Adsorption onto a Poly(2-methoxyethylacrylate) Surface by a Quartz Crystal Microbalance"Colloids and Surfaces A : Physicochemical and Engineering Aspects. 193.

M.Tanaka、A.Mochizuki、T.Motomura、K.Shimura、M.Onishi、Y.Okahata：“通过石英晶体微天平对聚（2-甲氧基乙基丙烯酸酯）表面上的蛋白质吸附进行原位研究”胶体和表面 A

H. Furusawa, Y. Kitamura, N. Hagiwara, T. Turimoto, and Y. Okahata: "Binding Kinetics of the Toroidal-Shaped PCNA to DNA Strands on a 27 MHz Quartz-Crystal Microbalance"Chem PhysChem.. No.5. 446-448 (2002)

H. Furusawa、Y. Kitamura、N. Hagiwara、T. Turimoto 和 Y. Okahata：“27 MHz 石英晶体微天平上环形 PCNA 与 DNA 链的结合动力学”Chem PhysChem.. No.5。

Y. Hoshino, S. Tajima, H. Nakayama, and Y. Okahata: "A RNA-aligned Film prepared from a RNA-Lipid Complex"Macromolecular rapid Commun.. 23. 253-255 (2002)

Y. Hoshino、S. Tajima、H. Nakayama 和 Y. Okahata：“由 RNA-脂质复合物制备的 RNA 排列薄膜”Macromolecular rapid Commun. 23. 253-255 (2002)

H.Matsuno, Y.Okahata: "Direct Monitoring of DNA Cleavages catalyzed by an ATP-dependent Deoxyribonuclease on a 27 MHz Quartz-Crystal Microbalance"Chem.Commun. 470-471 (2002)

H.Matsuno、Y.Okahata：“在 27 MHz 石英晶体微天平上直接监测 ATP 依赖性脱氧核糖核酸酶催化的 DNA 裂解”Chem.Commun。