Site specific sorting signals for the seed storage protein accumulation

种子储存蛋白积累的位点特异性分选信号

• 批准号: 12138207
• 负责人: TANAKA Kunisuke
• 金额: $ 24.58万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12138207/
• 关键词: rice; protein body; endosperm cell; prolamin; glutelin; signal peptide; protein transport; cDNA library

We studied the accumulation processes of storage proteins in the developing rice endosperm cells as a model system to understand the general mechanisms for the gene expression, the biosynthesis, and transport of plant storage proteins. The major objectives of this study are, 1) To clarify the differential mechanism of the endosperm cell, 2) To specify the targeting site of storage proteins on the ER membrane, and 3) To characterize the membranes of protein body type II and aleurone particle.As the result of 1), we isolated an Rcdl cDNA that is expressed specifically at the very early developing stage ( at the third day after flowering, starchy endosperm cell and aleurone cell are not yet differentiated).2) Prolamin synthesis was observed on the growing protein body I (PB-I) membrane and the adjacent area to the PB membrane. A Myc antibody was used in this investigation.3) The distribution of the tonoplast intrinsic protein ( TIP ) isoforms on the membrane of protein body II and aleurone particle indicated both organelles derived from vacuoles. The different combinations of the TIP isoforms on both particles indicated the different developmental processes are existing to from these organelles.

我们研究了开发的水稻胚乳细胞中储存蛋白的积累过程，作为一种模型系统，以了解基因表达，生物合成和植物储存蛋白的运输的一般机制。这项研究的主要目的是，1）阐明胚乳细胞的差异机制，2）指定ER膜上储存蛋白的靶向位点，以及3）表征蛋白质体型II型蛋白质和核龙颗粒的膜。作为1的结果），我们是1天的rcdl cdna（在rcdl cdna上均隔离开花（在启动的阶段），这是在启动的阶段（在启动的阶段（在启动的阶段），这是在开花的阶段（在启动的阶段（在启动）上（在第三个阶段，都在启动（在第三阶段），这是在启动的阶段（在三个月中都在开花。 2）在生长的蛋白体I（PB-I）膜以及与PB膜的相邻区域上观察到丙书蛋白合成。 3）在蛋白质体II的膜上，块状固有蛋白（TIP）同工型的分布和麦芽元颗粒的分布表明两个细胞器均来自液泡。两个粒子上的尖端同工型的不同组合表明，来自这些细胞器的不同发育过程存在。

项目成果

Furukawa, S.: "Distribution of storage proteins in low-glutelin rice seed determined using a fluorescent antibody."J. of Bioscience and Bioengineering. 96. 467-473 (2003)

Furukawa, S.：“使用荧光抗体测定低谷蛋白水稻种子中储存蛋白的分布。”J.

Okada, A.: "Accumulation of Japanese cedar pollen allergen, Cry j 1, in the protein body I of transgenic rice seeds using the promoter and singal sequence of glutelin GluB-1 gene."Mol. Breed.. 12. 61-70 (2003)

Okada, A.：“利用谷蛋白 GluB-1 基因的启动子和信号序列，在转基因水稻种子的蛋白体 I 中积累日本雪松花粉过敏原 Cry j 1。”

Urano, J.: "Molecular cloning and characterization of a rice dehydroascorbate reductase"FEES Letters. 466. 107-111 (2000)

Urano, J.：“水稻脱氢抗坏血酸还原酶的分子克隆和表征”费用快报。

田中國介: "植物細胞工学シリーズ「植物オルガネラの分化と多様性」"秀潤社. 7 (2002)

田中国介：“植物细胞工程系列‘植物细胞器的分化和多样性’’Shujunsha.7（2002）

Mino, M.: "Hydrogen peroxide functions as a cell death signal for hybrid lethality in the F_1 of Nicotiana gossei x N. tabacum."Plant Sci., in press. (2004)

Mino, M.：“过氧化氢在戈赛烟草 x 烟草的 F_1 中作为杂交致死的细胞死亡信号。”《植物科学》，出版中。