Gene regulation of superoxide dismutase genes and plat breeding for environmental stress tolerance

超氧化物歧化酶基因的基因调控及耐环境胁迫的植物育种

基本信息

批准号：
07456150
负责人：
TANAKA Kunisuke
金额：
$ 5.12万
依托单位：
Kyoto Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456150/
关键词：
rice environmental stess tolerant plant breeding superoxide dismutase plastidic oxygen stress active oxygen ascorbate peroxidase glutathione raductase アスコルビン酸パーオキシダーゼ酵素ストレス

项目摘要

To clarify the mechanisms of gene regulation of superoxide dismutase (SOD) genes in plant is the aim of this project. Results are expected useful for plant breeding of environmental stress tolerant plants. Through fulfillment of this project it became to understand the totally controlled gene regulation mechanisms of SODィイD2sィエD2. The research was extended to the isolations and analyses of genes that are tightly linked to the detoxification of active oxygen molecules cooperating with SODィイD2sィエD2.Rice plastidic Cu/Zn SOD cDNA was structurally analyzed and the gene regulation was also investigated this year. Although isolation of monocotyledonous plastidic Cu/Zn SOD cDNA from maize has been extensively tried in the USA, the isolation is still unsuccessful.Monocotyledonous plastidic Cu/Zn SOD cDNA was first isolated in this study from rice and the part of the regulation mechanisms of this gene was clarified. Gene regulation of rice plastidic Cu/Zn SOD gene is strictly regulated by light as mRNA of this gene is expressed at the very short time after light illumination. This is why this type of cDNAs has been difficult to isolate. All SOD molecules expected, ascorbate peroxidases, and glutathione reductase genes are now available. The gene regulations for the active oxygen scavenging enzymes will be totally understandable by the future investigations of this area.

为了阐明植物中超氧化物歧化酶（SOD）基因基因调节的机制是该项目的目的。预计结果可用于耐受性胁迫植物的植物育种。通过实现该项目，它成为了Sodii D2Sie D2的完全控制的基因调节机制。 The research was extended to the isolations and analyses of genes that are tightly linked to the detoxification of active oxygen molecules cooperating With SOD2sie D2.Rice plastidic Cu/Zn SOD cDNA was structurally analyzed and the gene regulation was also investigated this year.Although isolation of monocotyledonous plastidic Cu/Zn SOD cDNA from maize has been extensively tried in美国的分离仍然不成功。在这项研究中，首先是从大米中分离出的单子叶质体cu/Zn SOD cDNA，并且阐明了该基因的调节机制的一部分。水稻质体Cu/Zn SOD基因的基因调节严格受到光调节，因为该基因的mRNA在光照射后的短时间内表达。这就是为什么这种类型的cDNA很难分离的原因。现在都可以使用所有预期的SOD分子，抗坏血酸酯过氧化物酶和谷胱甘肽还原基因。未来对该领域的研究将完全理解活性氧气清除酶的基因调节。