800 MHz NMR spectrometer (for liquids, with cryogenic probes)

800 MHz NMR 波谱仪（用于液体，带低温探头）

• 批准号: 493682661
• 金额: --
• 依托单位: Carl von Ossietzky Universität Oldenburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/493682661?language=en
• 关键词: 800; MHz; NMR; spectrometer; liquids

At the Institute for Chemistry and Biology of the Marine Environment (University of Oldenburg) microbiological interactions and global element cycles are main research themes. For this research we engage in a voyage through scales, from molecules and single cells to ecosystems and global biogeochemical cycles. Processes occurring on the sub-micrometer scale in the ocean ultimately accumulate to planetary scale effects. Half of global photosynthetic production and respiration is carried out by unicellular life in the ocean. All organisms in the ocean excrete dissolved organic matter (DOM). Despite being a foundation for microbial life, the DOM reservoir contains more carbon than the entire biomass on land. The reason for this accumulation is unknown. DOM is the marine geometabolome, which is the entirety of small molecule metabolic products and their abiotic transformation products. The geometabolome is one of the most complex mixtures known, consisting of millions of compounds. A main focus of the planned NMR applications is the structural analysis of the marine geometabolome and its precursors, i.e. the exo- and endometabolomes of marine organisms. Conventional biochemical analysis solved the structure of only <1% of all compounds in the deep-sea geometabolome because it is a largely inseparable mixture. Currently, our main tool is ultrahigh-resolution mass spectrometry, combined with multivariate statistics and numerical modelling. To date, this approach yields the most comprehensive and detailed molecular data of the geometabolome. However, mass spectrometry provides only basic structural information. Isomers are not resolved, and the quantification of individual DOM constituents is usually impossible because we lack sufficient structural data and as such standards for quantification. High-field NMR overcomes many of these crucial limitations and yields highly complementary information to mass spectrometry. An intrinsic drawback of NMR spectroscopy, compared to ultrahigh-resolution mass spectrometry, is its low sensitivity and resolution. The large number of individual compounds of the geometabolome causes signal broadening and overlap, leading to unresolved signals in one-dimensional NMR spectra. Two- and three-dimensional high-field NMR is powerful in resolving discrete structural features even in complex mixtures. This detailed structural information is not accessible with any other analytical technique. Highest resolution, sensitivity and access to long instrument time are therefore main prerequisites for our research. The high-field NMR spectrometer will be embedded in a new “GeoMetabolomics Center” at the University of Oldenburg, which will be unique worldwide. We have a fair and open user concept through which 40% of the new facility will be available to external users from the marine and geosciences, but also from medicine, pharmacology, biochemistry, and other disciplines in the region and worldwide.

在海洋环境化学与生物学研究所（奥尔登堡大学）微生物相互作用和全球元素周期是主要研究主题。在这项研究中，我们通过尺度进行航行，从分子和单个细胞到生态系统和全球生物地球化学循环。在海洋中的亚微米量表上发生过程，最终加速到行星量表的效果。全球光合产生和呼吸的一半是通过海洋中的单细胞寿命进行的。海洋中的所有生物都极为溶解有机物（DOM）。尽管是微生物寿命的基础，但DOM保留的碳多于陆地上的整个生物质。这种积累的原因尚不清楚。 DOM是海洋的几何代谢组，它是小分子代谢产物及其非生物转化产物的全部。几何代谢组是已知最复杂的混合物之一，由数百万化合物组成。计划中的NMR应用的主要重点是对海洋几何代谢组及其前体的结构分析，即海洋生物的外态代谢组。传统的生化分析解决了深海几何代谢组中所有化合物中仅<1％的结构，因为它在很大程度上是不可分割的混合物。当前，我们的主要工具是超高分辨率的质谱法，结合了多元统计和数值建模。迄今为止，这种方法产生了几何代谢组的最全面，最详细的分子数据。但是，质谱仅提供基本的结构信息。异构体无法解决，并且通常不可能进行单个DOM构建体的数量，因为我们缺乏足够的结构数据，并且作为定量标准。高场NMR克服了许多关键局限性，并为质谱法获得了高度完善的信息。与超高分辨率的质谱法相比，NMR光谱的固有缺点是其低灵敏度和分辨率。几何代谢组的大量化合物会导致信号扩展和重叠，从而导致一维NMR光谱中未解决的信号。即使在复杂的混合物中，二维和三维的高场NMR也可以解决离散的结构特征。任何其他分析技术都无法访问此详细的结构信息。因此，最高的分辨率，敏感性和距离较长的仪器时间是我们研究的主要先决条件。高场NMR光谱仪将嵌入到奥尔登堡大学的新“几何代谢组学中心”中，该中心将是全球独特的。我们有一个公平，开放的用户概念，通过该概念，新工厂中有40％的新设施将提供来自海洋和地球科学的外部用户，以及该地区和全球范围内的医学，药理学，生物化学和其他学科。