Science of Sho based on a metabolism study in the bowels of glucosides of Kampo medicine

基于汉方药苷类肠内代谢研究的翔科学

基本信息

批准号：
16590564
负责人：
OKAMURA Nobuyuki
金额：
$ 0.96万
依托单位：
Fukuyama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590564/
关键词：
glycyrrhizin intestinal microflora biotransformation HPLC Kampo medicine prodrug 腸内フローラ腸管代謝

项目摘要

A study of glycyrrhizin metabolism using rat fecal suspensions showed that glycyrrhizin consumption significantly activated glycyrrhizin metabolism, with glycyrrhizin consumption significantly elevating glycyrrhizin metabolism in some rats (responders) but not in others (nonresponders). Since intestinal microflora are believed to be responsible for the difference between responders and nonresponders, an attempt was made to identify those factors associated with the response to glycyrrhizin consumption by investigating the effects of long-term consumption and fasting on glycyrrhizin metabolism. The results showed that long-term glycyrrhizin consumption and fasting caused nonresponder. rats to become responders. Since fasting was the most significant factor in the response to glycyrrhizin consumption, fasting appears to be one of the major facilitators of intestinal metabolism of glycyrrhizin. Given that this noninvasive and convenient metabolic study could ascertain the metabolism of prodrugs such as glycyrrhizin, the technique could also be useful for identifying responders to herbal and Chinese medicines. Furthermore, the relationship between the response to glycyrrhizin consumption and pseudoaldosteronism was investigated by comparing urinary potassium and blood pressure, but no significant differences in these parameters were observed between responders and nonresponders.

使用大鼠粪便悬浮液进行的甘草酸代谢研究表明，摄入甘草酸显着激活甘草酸代谢，摄入甘草酸可以显着提高一些大鼠（有反应者）的甘草酸代谢，但在其他大鼠（无反应者）中则不然。由于肠道微生物群被认为是造成反应者和无反应者之间差异的原因，因此尝试通过研究长期食用和禁食对甘草甜素代谢的影响来确定与甘草酸消耗反应相关的因素。结果显示，长期食用甘草酸和禁食会导致无反应。老鼠成为反应者。由于禁食是甘草甜素消耗反应中最重要的因素，禁食似乎是甘草甜素肠道代谢的主要促进因素之一。鉴于这种无创且方便的代谢研究可以确定甘草酸等前药的代谢，该技术也可用于识别草药和中药的反应者。此外，通过比较尿钾和血压来研究甘草酸消耗反应与假性醛固酮增多症之间的关系，但在反应者和非反应者之间没有观察到这些参数的显着差异。