The role of Sox 17 during mouse gut formation.

Sox 17 在小鼠肠道形成过程中的作用。

基本信息

批准号：
16590152
负责人：
KANAI Masami
金额：
$ 1.98万
依托单位：
Kyorin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590152/
关键词：
endoderm mouse development gut formation Sox17因子

项目摘要

The Sox (Sry-related HMG box) gene family was first identified through its homology to the high mobility group (HMG) box of the sex determining factor gene SRY. Members of the Sox family have been isolated in various vertebrate species, including 20 Sox genes in humans and mice. Members of this gene family encode transcription factors that regulate the specification of cell types and tissue differentiation in various developmental processes. Analyses of Sox17 expression have revealed activity in the visceral and definitive endoderm of post-implantation mouse embryos. In this project, we have studied that the role of Sox17 on the mouse endoderm derived gut formation. mRNA of Sox17 is expressed on definitive entire gut endoderm at first, and may function as a maintenance factor in prospective foregut, and differenaitation regulator at mid and hindgut. The later stage, mRNA of Sox17 is expressed on hepatic diverticum. The liver from the heterozygous embryo also shows the alteration of peripheral region of lobes. The major phenotype observed is the decrease of approximate weight of the liver. This is a preliminary data, but the effect of genetical backrgound to the hepato-insufficisncycy are necessary to be observed in the further study. Our observation strongly indicate that a critical role of Sox17 in the determination of the cell into the endoderm and its derivative organ in mammals. These findings lead us to the possibility that Sox17 is one of the key genes for the isolation of the endodermal stem cells and obtaining of the endodermal cells derived from ES cells in the human therapeutic research for the future.

首先通过其与性别确定因子基因SRY的高移动性组（HMG）盒子的同源性鉴定了Sox（SRY相关的HMG盒）基因家族。 Sox家族的成员已经在各种脊椎动物物种中分离出来，其中包括人类和小鼠的20个SOX基因。该基因家族的成员编码转录因子，这些因子调节各种发育过程中细胞类型和组织分化的规范。 SOX17表达的分析表明，在植入后小鼠胚胎的内脏和确定的内胚层中的活性。在这个项目中，我们研究了SOX17在小鼠内胚层得出的肠道形成中的作用。首先，SOX17的mRNA在确定的整个肠内内胚层上表达，并且可能是前瞻性前肢的维护因素，而在中间和后肠里则是不同的征膜调节剂。后期的sox17 mRNA在肝憩室上表达。来自杂合胚胎的肝脏还显示了裂片外围区域的改变。观察到的主要表型是肝脏重量的减小。这是一个初步数据，但是在进一步的研究中，必须观察到遗传背面对肝脏实体的影响。我们的观察结果强烈表明，SOX17在细胞中确定内胚层及其衍生物器官中的临界作用。这些发现使我们有可能Sox17是分离内胚层干细胞的关键基因之一，并且是在人类的未来治疗研究中获得源自ES细胞的内胚层细胞的可能性。