The role of Sox17 during mouse early endoderm to its delivertives

Sox17在小鼠早期内胚层及其分娩过程中的作用

• 批准号: 20590178
• 负责人: KANAI Masami
• 金额: $ 2.91万
• 依托单位: Tokyo Medical and Dental University (2010-2011)Kyorin University (2008-2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590178/
• 关键词: 内胚葉; 発生・分化; ノックアウトマウス; 腸管形成; 肝臓; 胆のう; 発生学; 形態形成学; 発生、分化

The definitive gut endoderm give rise to various cell-types constituting digestive tract, liver, pancreas and associated visceral endoderm. In order to examine primordium endoderm(9. 5 dpc) development, we have cultured them for 3 days, and then analyzed the development/maturation in each explant. We have examined that chimeric study between Sox17 null ES cells introducing to wild blastcyst and their DNA array. Our observation strongly indicate that a critical role of Sox17 in the determination of the cell into the endoderm and its derivative organ in mammals. These findings lead us to the possibility that Sox17is one of the key genes for the isolation of the endodermal stem cells and obtaining of the endodermal cells derived from ES cells in the human therapeutic research for the future.

确定的肠内胚层产生构成消化道，肝脏，胰腺和相关内脏内胚层的各种细胞类型。为了检查内胚层原始（9。5dpc）的开发，我们已经培养了3天，然后分析了每种外植体中的发育/成熟。我们已经检查了引入野生BlastCyst及其DNA阵列的Sox17 Null ES细胞之间的嵌合研究。我们的观察结果强烈表明，SOX17在细胞中确定内胚层及其衍生物器官中的临界作用。这些发现使我们可能是Sox17IS是分离内胚层干细胞的关键基因之一，并在人类的未来治疗研究中获得了源自ES细胞的内胚细胞。

项目成果

Sox17 function in mouse hepatopancreatic system

Sox17在小鼠肝胰系统中的功能

• 发表时间: 2009
• 作者: Uemura M;Kanai-Azuma M
• 通讯作者: Kanai-Azuma M

The membrane-spanning domain of CD98 heavy chain promotes alpha(v) beta3 integrin signals in human extravillous trophoblasts

CD98重链的跨膜结构域促进人绒毛外滋养细胞中的α(v)β3整合素信号

• 发表时间: 2008
• 期刊: Mol Endocrinol
• 作者: Kabir-Salmani M;Kanai-Azuma M
• 通讯作者: Kanai-Azuma M

Roles of definitive endoderm in establishment of left-right asymmetry in the mouse embryos

定形内胚层在小鼠胚胎左右不对称性建立中的作用

• 发表时间: 2010
• 作者: Ranajeet S Saund;Masami Kanai-Azuma
• 通讯作者: Masami Kanai-Azuma

SRY-dependent inducibility of SOX9 expression in developing mouse ovaries : A sexually bipotential population of granulose cells and its contribution to sexual plasticity in ovarian follicles.

发育中小鼠卵巢中 SOX9 表达的 SRY 依赖性诱导性：颗粒细胞的性双能群体及其对卵巢卵泡性可塑性的贡献。

• 发表时间: 2010
• 作者: Kyoko Harikae;間2名;Masami;Kanai-Azuma;以下4名
• 通讯作者: 以下4名

Spatiotemporal Specification of Gallbladder/bile duct progenitors in mouse foregut development.

小鼠前肠发育中胆囊/胆管祖细胞的时空规范。

• 发表时间: 2010
• 作者: Mami Uemura;間9名;Masami Kanai-Azuma;Yoshiakira Kanai
• 通讯作者: Yoshiakira Kanai