Applied Study of Anti-inflammatory Compounds from Mushrooms for Health Care

蘑菇抗炎成分的保健应用研究

基本信息

批准号：
16580085
负责人：
HIROTA Mitsuru
金额：
$ 1.79万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580085/
关键词：
anti-inflammation mushroom TPA Sarcodon scabrosus ケロウジシイタケ

项目摘要

Skin tumor promoters such as TPA induce edema on the skin with cell proliferation. Inhibitors of TPA-induced edema can be expected to suppress various physiological responses involving cell proliferation as well as inflammation. We have searched anti-inflammatory compounds from mushroom.1. In this research, we studied the relations between structures of sarcodonins and anti-inflammatory activity.1) From fruit body of Sarcodon scabrosus, we isolated sarcodonin B (19-deoxy-sarcodonin A) and 19- acylsarcodonin A.2) Sarcodonin A was chemically modified to obtain derivatives. From the structure-activity study of natural and synthetic derivatives, oxygen-containing functional groups at C19 and C14 positions is supposed to be important for presence of anti-inflammatory activity.3) Sarcodonin L was chemically modified to give its derivatives. Comparing their activities, we concluded that proper hydrophobicity is necessary to show the strong activity.4) On the basis of the structure-activity relationships of sarcodonin A derivatives, we synthesized anthranoyl-sarcodonin A with fluorescence. This compound is expected to be one of tools for studying the mechanisms of anti-inflammatory activity of sarcodonins.5) In collaboration study, we studied anti-tumor activity of sarcodonin A. Sarcodonin A showed specific anti-tumor activity to esophageal cancer cell in vitro and in vivo.2. We searched compounds with anti-inflammatory activity from mushrooms and found the activity Ganoderma neolaponicum and Daedalea dickinsii and Lentinula edodes. From mycelia of Ganoderma neo-japonicum and Daedalea dickinsii we isolated lanostane type of triterpenes, and from the fruiting body of mushroom Lentinula edodes, we isolated three anti-inflammatory compounds 3,7-bis(hydroxymethyl)-1-benzoxepin- 5(2H)-one, and its related compounds.

TPA 等皮肤肿瘤促进剂会随着细胞增殖而引起皮肤水肿。 TPA 诱导的水肿抑制剂有望抑制涉及细胞增殖和炎症的各种生理反应。我们从蘑菇中寻找到了抗炎化合物。1．在本研究中，我们研究了肌节蛋白的结构和抗炎活性之间的关系。1）从Sarcodon scabrosus子实体中，我们分离了肌节蛋白B（19-脱氧肌节蛋白A）和19-酰基肌节蛋白A。2）肌节蛋白A是经化学修饰得到衍生物。从天然和合成衍生物的结构活性研究来看，C19和C14位置的含氧官能团被认为对于抗炎活性的存在很重要。3)对Sarcodonin L进行化学修饰，得到其衍生物。比较它们的活性，我们得出结论，适当的疏水性是显示强活性所必需的。4)根据肌节素A衍生物的构效关系，我们合成了具有荧光功能的蒽酰肌节素A。该化合物有望成为研究肌节蛋白抗炎活性机制的工具之一。5)在合作研究中，我们研究了肌节蛋白A的抗肿瘤活性。肌节蛋白A在食管癌细胞中表现出特异性的抗肿瘤活性。体外和体内2．我们从蘑菇中搜索了具有抗炎活性的化合物，发现了灵芝、狄更斯菌和香菇的活性。从灵芝和大德莲的菌丝体中分离出羊毛甾烷型三萜类化合物，从蘑菇香菇的子实体中分离出三种抗炎化合物3,7-双(羟甲基)-1-苯并氧平- 5(2H) )-一及其相关化合物。