Identification and characterization of genes for cell polarity and asymmetric cell division in plants

植物细胞极性和不对称细胞分裂基因的鉴定和表征

• 批准号: 16570041
• 负责人: FUJITA Tomomichi
• 金额: $ 2.43万
• 依托单位: Hokkaido University (2005)National Institute for Basic Biology (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16570041/
• 关键词: plants; Physcomitrella patens; asymmetric cell division; gene expression; development; cell differentiation; cell polarity; stem cell; 不等分裂; ヒメツリガネゴケ; プロトプラスト; 完全長cDNA; 過剰発現; RNA干渉; 再生

Asymmetric cell division generates two different daughter cells. Although unequal distribution of mRNA or proteins has been known to play a pivotal role to specialize each daughter cell, such molecules in plants remain largely unknown. The moss, Physcomitrella patens assures a good system for the study of molecular mechanisms for asymmetric cell division. The protoplast divides asymmetrically to generate apical stem cell and differentiated protonemal, non-stem cell, thereafter the apical stem cell continues to divide asymmetrically to generate a row of differentiated protonemal cells. We have devised a systematic overexpression screening and identified 58 cDNAs as candidates for genes that are involved in asymmetric cell division. For those candidates, we made cDNA-citrine knock-in transgenic plants by using gene targeting technique to investigate protein localization during asymmetric cell division under a control of native promoters. We found several fusion proteins preferentially accumulated in apical stem cells, two of which were intracellularly localized within the apical cell.

不对称细胞分裂产生两个不同的子细胞。尽管已知 mRNA 或蛋白质的不均匀分布对于每个子细胞的特化发挥着关键作用，但植物中的此类分子仍然很大程度上未知。苔藓植物小立碗藓为研究不对称细胞分裂的分子机制提供了一个良好的系统。原生质体不对称分裂产生顶端干细胞和分化的原质非干细胞，此后顶端干细胞继续不对称分裂产生一排分化的原质细胞。我们设计了系统的过表达筛选，并鉴定了 58 个 cDNA 作为参与不对称细胞分裂的候选基因。对于这些候选者，我们通过使用基因打靶技术来研究在天然启动子控制下的不对称细胞分裂过程中的蛋白质定位，制备了 cDNA-黄水晶敲入转基因植物。我们发现几种融合蛋白优先在顶端干细胞中积累，其中两种融合蛋白位于顶端细胞的细胞内。

项目成果

Genes for the peptidoglycan synthesis pathway are essential for chloroplast division in moss

• DOI: 10.1073/pnas.0510693103
• 发表时间: 2006-04-25
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Machida, M;Takechi, K;Takano, H
• 通讯作者: Takano, H

Isolation of mutant lines with decreased numbers of chloroplasts per cell from a tagged mutant library of the moss Physcomitrella patens

• DOI: 10.1055/s-2005-837691
• 发表时间: 2005-05-01
• 期刊: PLANT BIOLOGY
• 影响因子: 3.9
• 作者: Hayashida, A;Takechi, K;Takano, H
• 通讯作者: Takano, H

New Frontiers in Bryology : Physiology, Molecular Biology and Functional Genomics

苔藓学新领域：生理学、分子生物学和功能基因组学

• 发表时间: 2004
• 作者: Fujita;T
• 通讯作者: T