Study of molecular mechanism of asymmetry in plant stem cells

植物干细胞不对称性分子机制研究

• 批准号: 23657027
• 负责人: FUJITA Tomomichi
• 金额: $ 2.5万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657027/
• 关键词: 植物ホルモン; 成長生理; 全能性; 植物; 非対称分配; ヒメツリガネゴケ; 転写因子; 細胞壁タンパク質; アブシジン酸

Asymmetric cell division is important for multicellular organisms to control their developmental program. Molecular mechanisms of asymmetric cell division are still largely unknown in plants. The moss, Physcomitrella patens provides a good system for studying the mode of cell division since the whole processes are easily observable at a single cell level.We identified ten of the fluorescent protein-tagged proteins were segregated asymmetrically during cell division, and found distinct types of uneven segregation are underlying during asymmetric cell division. We also found that either under a stress condition or upon abscisic acid treatment, the apical cell changed a cell division mode from asymmetry to symmetry. Then in order to understand a molecular mechanism for how the mode of cell division can be switched, we identified several genes, and further studied their function.

不对称细胞分裂对于多细胞生物控制其发育程序非常重要。植物中不对称细胞分裂的分子机制仍然很大程度上未知。苔藓植物 Physcomitrella patens 为研究细胞分裂模式提供了一个良好的系统，因为整个过程在单细胞水平上很容易观察到。我们鉴定了十种荧光蛋白标记蛋白在细胞分裂过程中不对称分离，并发现了不同的类型不均匀分离是不对称细胞分裂过程中的基础。我们还发现，无论是在应激条件下还是在脱落酸处理下，顶端细胞将细胞分裂模式从不对称改变为对称。然后，为了了解细胞分裂模式如何切换的分子机制，我们鉴定了几个基因，并进一步研究了它们的功能。

项目成果

Plant extracellular matrix protein regulates asymmetric and symmetric modes of cell division

植物细胞外基质蛋白调节细胞分裂的不对称和对称模式

• 作者: Tanaka R;Takabayashi A;Ito H and Tanaka A;土屋祐弥,中村康平,今井裕之,坂田洋一,Ralph Quatrano,長谷部光泰,藤田知道
• 通讯作者: 土屋祐弥,中村康平,今井裕之,坂田洋一,Ralph Quatrano,長谷部光泰,藤田知道

Cell wall proteins regulates asymmetric and symmetric modes of cell division

细胞壁蛋白调节细胞分裂的不对称和对称模式

• 发表时间: 2012
• 作者: Nakajima S;Ito H;Tanaka R;Tanaka A;Tomomichi Fujita
• 通讯作者: Tomomichi Fujita

The Selaginella genome identifies genetic changes associated with the evolution of vascular plants.

• DOI: 10.1126/science.1203810
• 发表时间: 2011-05-20
• 期刊: Science (New York, N.Y.)
• 作者: Banks JA;Nishiyama T;Hasebe M;Bowman JL;Gribskov M;dePamphilis C;Albert VA;Aono N;Aoyama T;Ambrose BA;Ashton NW;Axtell MJ;Barker E;Barker MS;Bennetzen JL;Bonawitz ND;Chapple C;Cheng C;Correa LG;Dacre M;DeBarry J;Dreyer I;Elias M;Engstrom EM;Estelle M;Feng L;Finet C;Floyd SK;Frommer WB;Fujita T;Gramzow L;Gutensohn M;Harholt J;Hattori M;Heyl A;Hirai T;Hiwatashi Y;Ishikawa M;Iwata M;Karol KG;Koehler B;Kolukisaoglu U;Kubo M;Kurata T;Lalonde S;Li K;Li Y;Litt A;Lyons E;Manning G;Maruyama T;Michael TP;Mikami K;Miyazaki S;Morinaga S;Murata T;Mueller-Roeber B;Nelson DR;Obara M;Oguri Y;Olmstead RG;Onodera N;Petersen BL;Pils B;Prigge M;Rensing SA;Riaño-Pachón DM;Roberts AW;Sato Y;Scheller HV;Schulz B;Schulz C;Shakirov EV;Shibagaki N;Shinohara N;Shippen DE;Sørensen I;Sotooka R;Sugimoto N;Sugita M;Sumikawa N;Tanurdzic M;Theissen G;Ulvskov P;Wakazuki S;Weng JK;Willats WW;Wipf D;Wolf PG;Yang L;Zimmer AD;Zhu Q;Mitros T;Hellsten U;Loqué D;Otillar R;Salamov A;Schmutz J;Shapiro H;Lindquist E;Lucas S;Rokhsar D;Grigoriev IV
• 通讯作者: Grigoriev IV

ヒメツリガネゴケにおけるCDKAの機能解析

三叶立碗藓CDKA的功能分析

• 作者: 石橋充浩;巻口勇馬;野田なつみ;日渡祐二;石川雅樹;鈴木穣;菅野純夫;長谷部光泰;藤田知道
• 通讯作者: 藤田知道

アブシジン酸により誘導される細胞分裂と細胞分化に関わる新奇因子の同定と解析

脱落酸诱导细胞分裂和分化的新因子的鉴定和分析

• 作者: 土屋祐弥;今井裕之;中村康平;坂田洋一;Ralph Quatrano,長谷部光泰;藤田知道
• 通讯作者: 藤田知道