FOR CLINICAL APPLICATION OF PANCREATIC ISLET TRANSPLANTATION-ANALYSIS OF THE PANCREATIC ISLETS GROWTH FACTOR USED MICRODISSECTION

胰岛移植的临床应用——显微切割法分析胰岛生长因子

基本信息

批准号：
14571116
负责人：
ANZAI Keizo
金额：
$ 2.24万
依托单位：
FUKUOKA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571116/
关键词：
PANCREATIC ISLET TRANSPLANTATION TYPE 1 DIABETES MELLITUS PROLIFERATING GENE OF PANCREATIC ISLET マイクロダイゼクション

项目摘要

At present, Islet transplantation is highlighted as one alternative of treatment for type 1 diabetes mellitus. In this study, we analyze the gene expression in the proliferating islets and intend to identify the main factors that cause the islet cells to proliferate, keeping insulin secretional capacity and glucose responsibility. We used the partial pancreatectomy mice as in vivo proliferation model of islets. One important advantage of this study is that we used the islets which proliferated and regenerated, keeping insulin secretional capacity and glucose responsibility in the physiological environment in vivo. Another is that we selectively recovered the proliferating islets using Laser Captured Microdissection (LCM). Using LCM, we can selectively, recover the part of the purpose from the tissue section affixed on the slide glass. by the laser. Actually, we selectively cut off the islets which proliferated in the partial pancreatectomy mice using the LCM, and extracted the total RNA, and then analyzed the expressing genes by the cDNA microarray. Reflecting the functional acceleration of islets in partial pancreatectomy mice, the gene expression such as glucagon were increased. At present, we are analyzing how these genes are concerned in the proliferation of islet.

目前，胰岛移植被强调为1型糖尿病的治疗方法。在这项研究中，我们分析了增殖胰岛中的基因表达，并打算确定导致胰岛细胞增殖的主要因素，保持胰岛素的分泌能力和葡萄糖责任。我们将部分胰腺切除术小鼠用作体内胰岛增殖模型。这项研究的一个重要优点是，我们使用了胰岛增殖和再生的胰岛，从而在体内生理环境中保持胰岛素的分泌能力和葡萄糖责任。另一个是，我们使用激光捕获的微分解（LCM）选择性地恢复了增殖的胰岛。使用LCM，我们可以选择性地从固定在玻璃玻璃上的组织部分中恢复部分目的。由激光。实际上，我们选择性切断了使用LCM在部分胰腺切除术小鼠中增殖的胰岛，并提取总RNA，然后通过cDNA微阵列分析表达基因。反映部分胰腺切除术小鼠胰岛的功能加速度，增加了基因表达（例如胰高血糖素）。目前，我们正在分析这些基因在胰岛增殖中的关注。