Investigation for tumor progression factors in pediatric lymphoma

小儿淋巴瘤肿瘤进展因素的调查

基本信息

批准号：
17591121
负责人：
NAKAGAWA Atsuko
金额：
$ 1.86万
依托单位：
National Research Institute for Child Health and Development
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591121/
关键词：
pediatric lymphoma Cox-2 FISH C-myc

项目摘要

Newly diagnosed 133 pediatric lymphomas were classified according to the WHO classification. To classify the lymphomas, HE stain and Immunoperoxidase stainings for CD3, CD4, CD8, CD 15, CD20, CD30, CD79a, EMA, GranzymeB, and Ki-67 were performed on paraffin-embedded specimens. Detection of EBV was done by in situ hybridization (ISH) with an oligoprobe for EBV-encoded small non-polyadeneylated RNAs (EBER).Cox-2 expression was detected by RT-PCR and immunohisochemistry. C-myc gene translocation was examined by FISH in 26 mature B-cell lymphomas (Burkitt lymphoma and diffuse large B-cell lymphoma). Nine of 10 Burkitt lymphoma cases showed c-myc gene split signals and eight cases had a t(8;14) translocation. In the diffuse large B-cell lymphoma cases, only one case out of nine cases showed c-myc gene split signals. All the 26 mature B-cell lymphomas were negative for EBER-ISH. Differentiation Burkitt lymphoma from diffuse large B-cell lymphoma is sometimes difficult by HE histology. According to the WHO classification, almost 100% (>95%) of Burkitt lymphoma cells should be positive for Ki-67. In the diffuse large B-cell lymphoma, the proliferating fraction is lower than the Burkitt lymphoma. C-myc translocation was also useful to distinguish the Burkitt lymphoma from the diffuse large B-cell lymphoma. Cox-2 expression was varied among histological types. Anaplastic large cell lymphoma (ALCL) expressed cox-2 frequently. Cox-2-positive ALCL was compared with the gene expression profiling of cox-2-negative ALCL, but no significant difference was seen. In ALCL, the expression of cyclin D3 and BCL-6 were high regardless of the cox-2 expression.

新诊断的133例小儿淋巴瘤按照WHO分类进行分类。为了对淋巴瘤进行分类，对石蜡包埋的标本进行了 CD3、CD4、CD8、CD 15、CD20、CD30、CD79a、EMA、GranzymeB 和 Ki-67 的 HE 染色和免疫过氧化物酶染色。通过原位杂交 (ISH) 检测 EBV 编码的小非聚腺苷酸化 RNA (EBER)。通过 RT-PCR 和免疫组织化学检测 Cox-2 表达。通过 FISH 检查 26 例成熟 B 细胞淋巴瘤（伯基特淋巴瘤和弥漫性大 B 细胞淋巴瘤）中的 C-myc 基因易位。 10 例伯基特淋巴瘤病例中有 9 例显示 c-myc 基因分裂信号，8 例存在 t(8;14) 易位。在弥漫性大B细胞淋巴瘤病例中，9例中只有1例显示c-myc基因分裂信号。所有 26 例成熟 B 细胞淋巴瘤的 EBER-ISH 均为阴性。有时难以通过 HE 组织学区分伯基特淋巴瘤与弥漫性大 B 细胞淋巴瘤。根据 WHO 分类，几乎 100%（>95%）的伯基特淋巴瘤细胞应呈 Ki-67 阳性。弥漫性大 B 细胞淋巴瘤的增殖分数低于伯基特淋巴瘤。 C-myc 易位也有助于区分伯基特淋巴瘤和弥漫性大 B 细胞淋巴瘤。 Cox-2 表达因组织学类型而异。间变性大细胞淋巴瘤 (ALCL) 频繁表达 cox-2。将 Cox-2 阳性 ALCL 与 cox-2 阴性 ALCL 的基因表达谱进行比较，但没有发现显着差异。在 ALCL 中，无论 cox-2 表达如何，细胞周期蛋白 D3 和 BCL-6 的表达均较高。

项目成果

期刊论文数量（19）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Antineoplastic agents for pediatric anaplastic large cell lymphoma : Vinblastine is the most effective in vitro.

小儿间变性大细胞淋巴瘤的抗肿瘤药物：长春花碱是体外最有效的。

DOI：
发表时间：
2005
期刊：
Leuk Lymphoma 46
影响因子：
0
作者：
Muto A;Nakagawa A;Shimomura Y;Kitagawa Y;Tsurusawa M
通讯作者：
Tsurusawa M

Is neuroendocrine cell differentiation detected using chromogranin A from patients with bone metastatic prostate cancer a prognostic factor for outcome?

使用嗜铬粒蛋白 A 检测骨转移性前列腺癌患者的神经内分泌细胞分化是否是结果的预后因素？

DOI：
发表时间：
2006
期刊：
Oncology Reports7 15
影响因子：
0
作者：
Yamada Y;Nakamura K;Aoki S;Taki T;Matsubara H;Sai S;Naruse K;Tibiume M;Katsuda R;Zennami K;Honda N;Nakagawa A;Ikeda H.
通讯作者：
Ikeda H.

治療中に白血病に移行したKlinefelter症候群合併anaplastic large cell lymphomaの1例

一例与克兰费尔特综合征相关的间变性大细胞淋巴瘤在治疗期间进展为白血病。