Effect of docosahexaenoic acid on the differentiation of neural steam cells

二十二碳六烯酸对神经蒸汽细胞分化的影响

• 批准号: 17590205
• 负责人: HASHIMOTO Michio
• 金额: $ 2.24万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590205/
• 关键词: Neural steam cells; Docosahexaenoic acid; Neurogenesis; Memory and learning; Dementia; 分化誘導能; 海馬; 学習・記憶; ラット

Docosahexaaenoic acid (DHA), one of the main structural lipids in the mammalian brain, plays crucial roles in the development and function of brain neurons. We previously reported that chronic administration of DHA enhances long-term memory in young and aged rats, and dietary DHA has preventive and ameliorative effects on the impairment of spatial cognition learning ability in amyloid b-infused rats. These findings suggest that DHA plays crucial roles in the development and function of the CNS throughout life. In this study, we examined the 1) effect of DHA on neural differentiation of neural stem cells (NSCs) in vivo and in vitro, and 2) on the expression of basic helix-loop-helix (bHLH) transcriptional factors of the cultured cells.<Methods> NSCs obtained from 15.5-day-old rat embryos were propagated as neurospheres and cultured under differential conditions with or without DHA. Total RNA was prepared from each culture with or without DHA, and was performed RT-PCR (Hes1, Hes5, neurog … More enin1, Mash1 or NeuroD).<Results and Conclusions> 1) DHA significantly increased the number of Tuj 1-positive neurons compared with the control on cultured NSCs, and the newborn neurons in the DHA groups were morphologically more mature than in the control, DHA significantly decreased the incorporation ratio of 5-bromo-2-deoxyuridine, the mitotic division marker, during the first 24h periods. Furthermore, dietary administration of DHA significantly increased the number of 5-bromo-2-deoxyuridine(+)/NeuN(+) newborn neurons in the granule cell layer of the dentate gyrus in adult rats. These results suggested that DHA effectively promotes nuerogenesis both in vitro and in vivo.2) Treatment of NSCs with DHA decreased the expression of Hes1 mRNA compared with that of control, suggesting that DHA stimulates neuronal differentiation by modulating expression levels of bHLH transcription factors.The present study suggests that DHA has the new property of modulating hippocampal function regulated by neurogenesis. Less

Docosahexaaenoic Acid（DHA）是哺乳动物大脑中主要的结构脂质之一，在脑神经元的发育和功能中起着至关重要的作用。我们先前曾报道过，长期给予DHA增强了年轻大鼠和老年大鼠的长期记忆，而饮食DHA对淀粉样蛋白B融合大鼠的空间认知学习能力的损害具有预防和改善作用。这些发现表明，DHA在CNS一生的发展和功能中起着至关重要的作用。在这项研究中，我们检查了1）DHA对体内和体外神经元干细胞（NSC）神经元分化的影响，以及2）对培养的细胞的基本螺旋 - 环螺旋（BHLH）转录因子的表达。从每种培养物中制备有或没有DHA的每种培养的RNA，并进行RT-PCR（HES1，HES5，NEUROG…更多ENIN1，MASH1或NEUROD）。<结果和结论> 1）DHA显着增加了Tuj 1阳性神经元的数量，与培养的NSC的对照组相比，与新生儿的神经元相比，与新生儿的控制相比，与新生儿有关，而与新生的神经元相比，该型神经元与医学上的神经元相比，是多个神经元的神经元的神经元的，而不是神学的神经元，而DHA的神经元比较有效期，而DHA的神经元比较有效地神经元，而DHA的神经元比学家的神经元更加多种神经元的神经元，那么dha是多个神经元的神经元的神经元的数字。在最初的24H时期，有丝分裂分裂标记的5-溴-2-脱氧尿苷的比率。此外，DHA的饮食施用显着增加了成年大鼠齿状回的颗粒细胞层中5-溴-2-脱氧尿苷（+）/neun（+）新生神经元的数量。这些结果表明，DHA有效地促进了体外和体内的刺激性。2）NSC用DHA处理与对照组相比，DHA对HES1 mRNA的表达降低，这表明DHA通过调节BHLH转录因子的调节水平刺激神经元分化。本研究表明，DHA表明DHA具有模块化依赖的HippocAmpals farimpoCampAmpAndal功能。较少的

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神経再生促進剤

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• 发表时间: 2005
• 期刊: 島根県立看護短期大学紀要 11
• 作者: 山下一也;他
• 通讯作者: 他

Myosin light chain kinase and Rho-kinase participate in P2Y receptor-mediated acceleration of permeability through the endothelial cell layer.

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• 发表时间: 2005
• 期刊: Journal of Pharmacy and Pharmacology 57
• 作者: Naoko Tanaka;et al.
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• 发表时间: 2006
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