On the role of subtypes of muscarinic receptors in the inner ear

内耳毒蕈碱受体亚型的作用

• 批准号: 15591798
• 负责人: ITO Ken
• 金额: $ 2.3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591798/
• 关键词: muscarinic receptor; subtype; knock-out mice; patch clamp; spiral ganglion; vestibular ganglion; dissociated cells; 聴性脳幹反応(ABR); 内耳単離細胞

This year, we continued our research on the basis of what we have established during the past two years. However, we encountered two troubles at the beginning of the year. First, because of epidemic problems, supply of the knock-out animals was disconnected for about half a year. The stable supply was re-established in November and we resumed the experiments. Secondly, in the meantime, a competing laboratory in the States reported that their mice lacking muscarinic receptors (product independent of our knock-outs) had almost normal hearing and similar susceptibility to ototoxic agents (Maison SF, et al. 2005).However, we made progress in two points. First, we revised the computer software to record OAEs, which was a pending problem from the last year, and enabled recording DPOAEs in animals as well as in humans. Secondly, considering the situation described above, we tried to shift the axis of our research in the inner ear from the auditory system to the vestibular system and finally w … More ere able to record action potentials of vestibular ganglion cells in rodents on the whole-cell patch clamp mode. We also revealed the expression of mRNAs for muscarinic receptors in vestibular ganglion cells by means of DNA arrays.Planned studies in the near future :1) We still continue the experiments on comparative studies on susceptibility to extraordinary noise and to ototoxic drugs using knock-out and wild-type mice. This is because the results may differ from those from the USA group, since the knock-out mice were independently produced.2) Since the existence of muscarinic receptors on the vestibular ganglion cells in rodents was indicated, we plan first to investigate modulation of excitability of neurons by muscarinic receptors and then to reveal the difference of neuronal firing patterns between knock-out and wild-type mice. Moreover, by investigating the difference in contribution of ionic channels on action potential formation, the contribution of muscarinic receptors to developmental processes of vestibular ganglion cells will be deduced. Less

今年，在过去的两年中。 Y在州报告了他们缺乏毒蕈碱受体（与我们的敲除的产物）几乎正常的听力，并且类似于耳毒剂（Maison SF等，2005）记录OAE，这是去年的一个待处理问题，并使DPOAES和人类都可以记录。通过DNA阵列记录啮齿动物中前庭神经节细胞的作用电位。使用敲除和野生型小鼠的耳毒药物可能与美国毒蕈碱神经节细胞上的毒蕈碱受体的存在不同。 - 型小鼠

项目成果

Actions of subtype-specific purinngic ligands on rat spiral ganglin neurons

亚型特异性嘌呤配体对大鼠螺旋神经节神经元的作用

• 发表时间: 2004
• 期刊: Acta Otolaryngol(Stockh) S553
• 作者: Ken Ito;Shinichi Iwasaki;et al.
• 通讯作者: et al.

Shinichi Iwasaki, Ken Ito, et al.: "Does external analitory canal coordinate development of malleal manubrium in humans?"Acta Otolaryngol Head Neck Surg. (in press).

Shinichi Iwasaki、Ken Ito 等人：“外肛管是否协调人类踝骨柄的发育？”Acta Otolaryngol Head Neck Surg。

Aging and Progression of Hearing Loss in Mice Locking Muscarinic Acetylcholine Recepeor Subtype3

锁定毒蕈碱乙酰胆碱受体亚型 3 的小鼠的衰老和听力损失进展

• 发表时间: 2005
• 期刊: Abstvaets of the Annwal Research Meeting, Association for Research in Otolanyngology 28
• 作者: Ken Ito.Shinichi Iwasaki;Minoru Matsui
• 通讯作者: Minoru Matsui

Aging and progression of hearing loss in mice lacking muscarinic acetylcholine receptor sybtype 3

缺乏毒蕈碱乙酰胆碱受体sybtype 3的小鼠的衰老和听力损失的进展

• 发表时间: 2005
• 期刊: Abstracts of the Annual Research Meeting, Association for Research in Otolaryngology 28
• 作者: Ito K;Iwasaki S;Matsui M
• 通讯作者: Matsui M

Ken Ito, Shinichi Iwasaki, et al.: "Actions of subtype-specific purinerjic ligands on rat spiral ganglion neurons."Acta Otolaryngol (Stockh). (in press).

Ken Ito、Shinichi Iwasaki 等人：“亚型特异性嘌呤配体对大鼠螺旋神经节神经元的作用。”Acta Otolaryngol (Stockh)。